Relationship between gingival crevicular fluid and serum antibody titers in young adults with generalized and localized periodontitis
Relationship between gingival crevicular fluid and serum antibody titers in young adults with generalized and localized periodontitis
复制标题
青壮年广泛性和局限性牙周炎龈沟液与血清抗体滴度的关系
作者：
J. Tew;D. R. Marshall;J. Burmeister;R. Ranney
本研究的目的是确定青少年牙周炎和重度牙周炎患者血清中抗体浓度与龈沟液（GCF）中抗体浓度之间的关系。大多数用于定量抗体的抗原是从与青少年牙周炎或严重牙周炎相关的细菌中获得的。我们进一步研究了不同牙周部位之间抗体滴度的变化，以及GCF中抗体的局部来源。抗体滴度，总免疫球蛋白G（IgG）和人血清白蛋白测定灵敏的放射免疫分析。血清与GCF抗体的关系复杂。在临床状态相似的不同研究中心，发现了同一受试者的人与人之间的差异和显著差异。不仅与牙周生物反应的抗体存在位点间差异，抗破伤风类毒素、总IgG甚至人血清白蛋白也存在位点间差异。一般来说，变异性在于GCF水平相对于血清水平的降低程度。但龈沟液中抗牙龈拟杆菌和抗伴放线放线杆菌抗体水平常高于血清水平。当计算每毫克人血清白蛋白的血清和GCF中的抗体滴度时，GCF中抗体的大部分明显降低消失。除B外，所有微生物的血清抗体与GCF抗体的比值接近1。gingivalis和A.放线菌伴放线菌Y4的表达显著升高。此外，GCF中每毫克人血清白蛋白的IgG水平约为血清水平的两倍。我们认为人血清白蛋白反映了血清对GCF的贡献，因此我们将GCF中每毫克白蛋白IgG水平的增加归因于局部合成。似乎抗B。牙龈炎和抗A.伴随放线菌是这种局部抗体合成的重要部分，因为大多数患有严重或青少年牙周炎的血清阳性患者至少有一个部位升高，并且在许多部位升高的幅度很大。与GCF中抗体水平与血清水平相似的部位相比，产生抗体升高的部位在可探测深度或附着水平的临床参数方面没有明显差异。GCF中抗体升高可能与疾病活动性的变化有关，这些变化无法通过常规临床措施检测到。
The objective of the present study was to determine the relationship between concentrations of antibodies in serum and those in gingival crevicular fluid (GCF) of patients with juvenile periodontitis and severe periodontitis. Most antigens used to quantitate antibodies were obtained from a panel of bacteria associated with juvenile periodontitis or severe periodontitis. We further investigated variation in antibody titer among different periodontal sites and the extent to which antibody in GCF is locally derived. Titers of antibody, total immunoglobulin G (IgG), and human serum albumin were determined with sensitive radioimmunoassays. The relationship between serum and GCF antibody was complex. Both person-to-person variability and marked variability within the same subject were found among different sites of similar clinical status. The site-to-site variability was found not only for antibody reactive with periodontal organisms, but also for antitetanus toxoid, total IgG, and even human serum albumin. Generally the variability was in the degree of depression of the level in GCF relative to that in serum. However, anti-Bacteroides gingivalis and anti-Actinobacillus actinomycetemcomitans in GCF often exceeded the level in serum. When antibody titers in serum and GCF were calculated per milligram of human serum albumin, most of the apparent depressions of antibody in GCF disappeared. The ratio of antibody in serum to that in GCF approached unity for all organisms except B. gingivalis and A. actinomycetemcomitans Y4, which were markedly elevated. Furthermore, the level of IgG per milligram of human serum albumin in GCF was about twice the level in serum. We believe that human serum albumin reflects serum contribution to the GCF, and we therefore attribute the increased level of IgG per milligram of albumin in GCF to local synthesis. It appears that anti-B. gingivalis and anti-A. actinomycetemcomitans represent an important portion of this local antibody synthesis, since most seropositive patients with severe or juvenile periodontitis had at least one site elevated, and the magnitudes of the elevations were large in many sites. Those sites yielding elevated antibody exhibited no obvious differences in clinical parameters of probeable depth or attachment level as compared with sites in which antibody levels in GCF were similar to serum levels. Elevated antibody in GCF may relate to changes in disease activity that are not detectable by usual clinical measures.
青少年牙周炎、慢性牙周炎和牙周健康受试者的放线放线杆菌伴生放线杆菌抗体滴度比较。
影响因子：
6.7
作者：
Listgarten,MA;Lai,CH;Evian,CI
通讯作者：
Evian,CI
龈沟液针对口腔微生物的抗体。
影响因子：
3.5
作者：
Ebersole,JL;Taubman,MA;Smith,DJ;Goodson,JM
通讯作者：
Goodson,JM
体液免疫反应和人类牙周病的诊断。
影响因子：
3.5
作者：
Ebersole,JL;Taubman,MA;Smith,DJ;Socransky,SS
通讯作者：
Socransky,SS