Mouse placental cells secrete soluble leptin receptor (sOB-R): cAMP inhibits sOB-R production.
Mouse placental cells secrete soluble leptin receptor (sOB-R): cAMP inhibits sOB-R production.
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小鼠胎盘细胞分泌可溶性瘦素受体 (sOB-R)：cAMP 抑制 sOB-R 的产生。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Y. Murata
中科院分区：
文献类型：
--
作者：
M. Yamaguchi;T. Murakami;Y. Yasui;S. Otani;M. Kawai;K. Kishi;H. Kurachi;K. Shima;T. Aono;Y. Murata
本研究旨在鉴定小鼠胎盘是否分泌可溶性OB-R（sOB-R），并寻找其表达的调控因子。从胎盘和蜕膜中提取总RNA，通过北方印迹分析评估OB-R表达。蜕膜不表达OB-R mRNA。而OB-R mRNA在妊娠第13天胎盘组织中就有表达，随后表达逐渐增加，至妊娠第17天达高峰。培养妊娠第12天的小鼠胎盘细胞，通过北方印迹分析评估OB-R基因表达。OB-R mRNA表达从培养第2天开始检测到，并在培养第3天达到高峰。为了确定胎盘细胞是否释放sOB-R，对培养的胎盘细胞的上清液进行Western印迹分析。在培养的第二天在培养基中检测到sOB-R，并且sOB-R释放增加直到培养的第四天。添加瘦素对OB-R mRNA的表达无影响。然而，8-溴cAMP抑制OB-R mRNA的稳态水平和sOB-R蛋白的量在介质中的剂量和时间依赖性的方式。这些结果表明，滋养层细胞分化，表达和释放sOB-R在体内和体外，cAMP是几个有效的调节sOB-R分泌的小鼠胎盘。
The aims of this study were to identify whether mouse placenta secretes soluble OB-R (sOB-R) and to find the regulating factor of OB-R expression. Total RNAs were extracted from placenta and decidua, and OB-R expression was assessed by Northern blot analysis. Decidua did not express OB-R mRNA. However, OB-R mRNA expression was detectable in the placenta on day 13 of pregnancy, and then it increased and reached a peak on day 17 of pregnancy. Mouse placental cells from day 12 of pregnancy were cultured and OB-R gene expression was assessed by Northern blot analysis. OB-R mRNA expression was detectable from the second day of culture and reached a peak on the third day of culture. To determine whether placental cells release sOB-R, supernatant of cultured placental cells was subjected to Western blot analysis. sOB-R was detected in the medium by the second day of culture and sOB-R release increased up to the fourth day of culture. Addition of leptin to the medium did not affect expression of OB-R mRNA. However, 8-bromo cAMP inhibited both steady-state levels of OB-R mRNA and the amount of sOB-R protein in the medium in a dose- and time-dependent manner. These results suggest that trophoblast cells differentiate, express, and release sOB-R both in vivo and in vitro and that cAMP is one of several potent regulators of sOB-R secretion by the mouse placenta.
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DOI：
10.1073/pnas.89.23.11396
发表时间：
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发表时间：
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