An inverse relationship between ceramide synthesis and clinical severity in patients with psoriasis.
An inverse relationship between ceramide synthesis and clinical severity in patients with psoriasis.
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DOI：
10.3346/jkms.2004.19.6.859
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发表时间：
2004-12
影响因子：
4.5
通讯作者：
Kim NI
中科院分区：
文献类型：
--
作者：
Cho Y;Lew BL;Seong K;Kim NI
神经酰胺在维持表皮屏障中起主要作用。据推测，神经酰胺的消耗与表皮屏障功能的破坏有关，导致银屑病患者出现干燥和炎症的临床表现。本研究的目的是确定银屑病患者表皮神经酰胺合成水平与临床严重程度之间的关系。从银屑病患者的皮损和非皮损表皮中获得的样品与[14 C]丝氨酸（神经酰胺合成的引发剂）孵育。使用高效薄层色谱法分级分离总神经酰胺，并测量放射性。根据银屑病面积和严重程度指数评分系统对银屑病的临床严重程度进行分级。银屑病患者皮损表皮神经酰胺合成水平明显低于正常表皮，并与临床严重程度呈负相关。目前的研究结果表明，神经酰胺合成水平的下降，在表皮有助于银屑病的临床严重程度。
Ceramides play major roles in maintaining the epidermal barrier. It has been sus-pected that the depletion of ceramides, associated with disrupted barrier function in the epidermis, leads to the clinical manifestation of dryness and inflammation seen in patients with psoriasis. The aim of the present study was to determine the relation-ship between the level of ceramide synthesis in the epidermis and the clinical severity in patients with psoriasis. Samples from lesional and unlesional epidermis obtained from psoriasis patients were incubated with [14C]serine, an initiator of ceramide syn-thesis. otal ceramide was fractionated using high performance thin layer chromato-graphy, and the radioactivity was measured. The clinical severity of psoriasis was graded according to the psoriasis area and severity index scoring system. The level of ceramide synthesis in the lesional epidermis of patients was significantly lower than that in the unlesional epidermis and bore a negative correlation with the clinical severity of psoriasis. The present results suggest that the decreased level of ceramide synthesis in the epidermis contributes to the clinical severity of psoriasis.
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影响因子：
6.5
作者：
IMOKAWA, G;ABE, A;HIDANO, A
通讯作者：
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