Controlled human infection with Neisseria lactamica in late pregnancy to measure horizontal transmission and microbiome changes in mother-neonate pairs: a single-arm interventional pilot study protocol.
Controlled human infection with Neisseria lactamica in late pregnancy to measure horizontal transmission and microbiome changes in mother-neonate pairs: a single-arm interventional pilot study protocol.
复制标题
DOI：
10.1136/bmjopen-2021-056081
复制
发表时间：
2022-05-18
期刊：
影响因子：
2.9
通讯作者：
Read, Robert C.
中科院分区：
文献类型：
--
作者：
Theodosiou, Anastasia A.;Laver, Jay R.;Dale, Adam P.;Cleary, David W.;Jones, Christine E.;Read, Robert C.
婴儿上呼吸道微生物群部分来自母体呼吸道，某些微生物群与感染和呼吸道疾病风险的改变有关。内酰胺奈瑟菌是幼儿常见的咽部疾病，与脑膜炎奈瑟菌的携带和侵袭性疾病减少有关。经鼻接种N.内酰胺安全且可重复地减少N.健康成人中的脑膜炎菌群。我们建议孕妇接种N.研究目的是确定出生后是否发生新生儿咽部定植，并观察产后1个月内母婴对的微生物组演变。20名健康孕妇接受N.在妊娠36-38周时，使用内酰胺菌（野生型菌株Y 92 -1009）。将从产后1个月以上的母婴对中采集上呼吸道样本以及可选的母乳、脐带血和婴儿静脉血样本。我们将评估安全性，N。内酰胺定殖（通过靶向PCR）和纵向微进化（通过全基因组测序）以及微生物组进化（通过16 S rRNA基因测序）。本研究已获得伦敦中央研究伦理委员会的批准（21/PR/0373）。研究结果将尽快发表在同行评审的开放获取期刊上。NCT 04784845。
Infant upper respiratory microbiota are derived partly from the maternal respiratory tract, and certain microbiota are associated with altered risk of infections and respiratory disease. Neisseria lactamica is a common pharyngeal commensal in young children and is associated with reduced carriage and invasive disease by Neisseria meningitidis. Nasal inoculation with N. lactamica safely and reproducibly reduces N. meningitidis colonisation in healthy adults. We propose nasal inoculation of pregnant women with N. lactamica, to establish if neonatal pharyngeal colonisation occurs after birth, and to characterise microbiome evolution in mother–infant pairs over 1 month post partum. 20 healthy pregnant women will receive nasal inoculation with N. lactamica (wild type strain Y92-1009) at 36–38 weeks gestation. Upper respiratory samples, as well as optional breastmilk, umbilical cord blood and infant venous blood samples, will be collected from mother–infant pairs over 1 month post partum. We will assess safety, N. lactamica colonisation (by targeted PCR) and longitudinal microevolution (by whole genome sequencing), and microbiome evolution (by 16S rRNA gene sequencing). This study has been approved by the London Central Research Ethics Committee (21/PR/0373). Findings will be published in peer-reviewed open-access journals as soon as possible. NCT04784845.
登录
查看更多内容
影响因子：
168.9
作者：
Laxminarayan, Ramanan;Matsoso, Precious;Davies, Sally
通讯作者：
Davies, Sally
影响因子：
9.4
作者：
Bas, Evrim Kiray;Bulbul, Ali;Nuhoglu, Asiye
通讯作者：
Nuhoglu, Asiye
影响因子：
3.1
作者：
Bennett, JS;Griffiths, DT;Maiden, MCJ
通讯作者：
Maiden, MCJ
DOI：
10.1164/rccm.201703-0554oc
发表时间：
2017-12-15
影响因子：
24.7
作者：
Bosch, Astrid A T M;de Steenhuijsen Piters, Wouter A A;Bogaert, Debby
通讯作者：
Bogaert, Debby
影响因子：
16.6
作者：
Thorsen, Jonathan;Rasmussen, Morten A.;Bisgaard, Hans
通讯作者：
Bisgaard, Hans