Network for Pancreatic Organ Donors with Diabetes (nPOD): developing a tissue biobank for type 1 diabetes.
Network for Pancreatic Organ Donors with Diabetes (nPOD): developing a tissue biobank for type 1 diabetes.
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DOI：
10.1002/dmrr.2316
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发表时间：
2012-10
影响因子：
8
通讯作者：
Atkinson, Mark
中科院分区：
文献类型：
--
作者：
Campbell-Thompson, Martha;Wasserfall, Clive;Kaddis, John;Albanese-O'Neill, Anastasia;Staeva, Teodora;Nierras, Concepcion;Moraski, Jayne;Rowe, Patrick;Gianani, Roberto;Eisenbarth, George;Crawford, James;Schatz, Desmond;Pugliese, Alberto;Atkinson, Mark
糖尿病胰腺器官捐献者网络的建立是为了从具有不同1型糖尿病（T1D）风险水平的尸体器官捐献者中恢复和表征胰腺和相关器官。这些生物标本可供研究者进行合作研究，旨在解决与TID自然史和发病机制相关的问题。器官供体包括T1D患者（新发至长期）、非糖尿病自身抗体阳性受试者、非糖尿病对照和患有β细胞功能相关疾病的个体。胰腺恢复和运输符合移植级标准。回收的其他样品包括血清、全血、脾脏、胰腺和非胰腺淋巴结。对生物标本进行处理，以获得冻存细胞、固定石蜡和新鲜冷冻组织块以及快速冷冻样本。还测定了T1D自身抗体、C肽水平和高危等位基因的高分辨率HLA基因分型。已经招募了160多名供体（年龄从1天到> 90岁）。沿着质量管理体系建立了标准操作规程。通过开放的在线信息系统共享供体人口统计学、实验室测定和组织病理学表征。生物标本分发给60多名研究人员。nPOD计划为研究者提供了高质量的生物标本，而无需支付任何费用。强调合作和开放数据共享，以最大限度地发挥每个捐助者的研究潜力。在初步成功的基础上，nPOD计划正在扩大，以从欧洲国家回收与T1D发病机制和并发症相关的其他器官（PanFin网络）。
The Network for Pancreatic Organ donors with Diabetes was established to recover and characterize pancreata and related organs from cadaveric organ donors with various risk levels for type 1 diabetes (T1D). These biospecimens are available to investigators for collaborative studies aimed at addressing questions related to TID natural history and pathogenesis. Organ donors included T1D patients (new-onset to long-term), non-diabetic autoantibody positive subjects, non-diabetic controls, and individuals with disorders relevant to β-cell function. Pancreas recovery and transport met transplant-grade criteria. Additional samples recovered included serum, whole blood, spleen, and pancreatic and non-pancreatic lymph nodes. Biospecimens were processed for cryopreserved cells, fixed paraffin and fresh frozen blocks, and snap frozen samples. T1D autoantibodies, c-peptide levels, and high-resolution HLA genotyping for risk alleles were also determined. Over 160 donors have been enrolled (ages of 1 day to > 90 years). Standard operating procedures were established along with a quality management system. Donor demographics, laboratory assays, and histopathological characterizations were shared through an open online informatics system. Biospecimens were distributed to more than 60 investigators. The nPOD program provides access to high quality biospecimens without cost to investigators. Collaborations and open data sharing are emphasized to maximize research potential of each donor. Based on initial successes, the nPOD program is expanding to recover additional organs relevant to T1D pathogenesis and complications from European countries (PanFin network).
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影响因子：
7.7
作者：
Keenan HA;Sun JK;Levine J;Doria A;Aiello LP;Eisenbarth G;Bonner-Weir S;King GL
通讯作者：
King GL
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
12.8
作者：
Maniatis, AK;Yu, LP;Eisenbarth, GS
通讯作者：
Eisenbarth, GS
影响因子：
16.2
作者：
Orban T;Sosenko JM;Cuthbertson D;Krischer JP;Skyler JS;Jackson R;Yu L;Palmer JP;Schatz D;Eisenbarth G;Diabetes Prevention Trial-Type 1 Study Group
通讯作者：
Diabetes Prevention Trial-Type 1 Study Group
影响因子：
7.7
作者：
In't Veld, Peter;Lievens, Dirk;Pipeleers, Daniel
通讯作者：
Pipeleers, Daniel