Quantification of C(60)-induced membrane disruption using a quartz crystal microbalance.
Quantification of C(60)-induced membrane disruption using a quartz crystal microbalance.
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使用石英晶体微平衡对C（60）诱导的膜破坏进行定量。
DOI：
10.1039/c7ra13690k
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发表时间：
2018-03-05
期刊：
影响因子：
3.9
通讯作者：
Jiang, Wei
中科院分区：
文献类型：
--
作者：
Zeng, Yuxuan;Wang, Qi;Zhang, Qiu;Jiang, Wei
富勒烯C60纳米颗粒与细胞膜的直接接触是其细胞毒性的机制之一。在这项研究中，C60纳米粒子对脂质膜的影响进行了研究。以巨单层囊泡（GUV）为模型细胞膜，观察C60对GUV膜的破坏作用。C60 NP破坏了带正电的GUV，但不破坏带负电的囊泡，证实了静电力的作用。为了定量C60在膜上的粘附和诱导的膜破裂，使用支撑脂质双层（SLB）和一层小单层囊泡（SUV）覆盖石英晶体微天平（QCM）的传感器。SLB上的质量变化（ΔmSLB）是由膜上的C60粘附引起的，而SUV层上的质量变化（ΔmSUV）是C60粘附（质量增加）和SUV破坏（质量损失）的综合结果。沉积C60后，SLB的表面积（ASLB）远小于SUV的表面积（ASUV），但ΔmSLB大于ΔmSUV，表明C60 NPs对膜有明显的破坏作用。因此，建立了一种新的方法来量化NP诱导的膜破坏程度，使用ΔmSUV/ΔmSLB和ASUV/ASLB的值。通过这种方式，C60可以与其他类型的NP进行比较，以了解哪一种导致更严重的膜破坏。此外，C60 NPs引起的膜相变化可忽略不计，表明膜凝胶化不是C60 NPs的细胞毒性机制。该研究为预测富勒烯纳米颗粒对环境的危害以及评价不同纳米颗粒对膜的损伤程度提供了重要信息。富勒烯C60纳米颗粒由于静电力粘附在脂质膜上并导致膜破裂。
Direct contact between fullerene C60 nanoparticles (NPs) and cell membranes is one of mechanisms for its cytotoxicity. In this study, the influence of C60 NPs on lipid membranes was investigated. Giant unilamellar vesicles (GUVs) were used as model cell membranes to observe the membrane disruption after C60 exposure. C60 NPs disrupted the positively charged GUVs but not the negatively charged vesicles, confirming the role of electrostatic forces. To quantify the C60 adhesion on membrane and the induced membrane disruption, a supported lipid bilayer (SLB) and a layer of small unilamellar vesicles (SUVs) were used to cover the sensor of a quartz crystal microbalance (QCM). The mass change on the SLB (ΔmSLB) was caused by the C60 adhesion on the membrane, while the mass change on the SUV layer (ΔmSUV) was the combined result of C60 adhesion (mass increase) and SUV disruption (mass loss). The surface area of SLB (ASLB) was much smaller than the surface area of SUV (ASUV), but ΔmSLB was larger than ΔmSUV after C60 deposition, indicating that C60 NPs caused remarkable membrane disruption. Therefore a new method was built to quantify the degree of NP-induced membrane disruption using the values of ΔmSUV/ΔmSLB and ASUV/ASLB. In this way, C60 can be compared with other types of NPs to know which one causes more serious membrane disruption. In addition, C60 NPs caused negligible change in the membrane phase, indicating that membrane gelation was not the mechanism of cytotoxicity for C60 NPs. This study provides important information to predict the environmental hazard presented by fullerene NPs and to evaluate the degree of membrane damage caused by different NPs. Fullerene C60 NPs adhere on lipid membrane due to electrostatic force and cause membrane disruption.
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影响因子：
3.7
作者：
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通讯作者：
Shaitan, Konstantin V.
影响因子：
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作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
ROSEN, HJ