Genomic Diversity of Group A Rotavirus Strains Infecting Humans in Eastern India
Genomic Diversity of Group A Rotavirus Strains Infecting Humans in Eastern India
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印度东部感染人类的 A 组轮状病毒株的基因组多样性
DOI：
10.1128/jcm.40.1.146-149.2002
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发表时间：
2002
影响因子：
9.4
通讯作者：
T. Naik
中科院分区：
文献类型：
--
作者：
Soma Das;Adrish Sen;G. Uma;V. Varghese;Susmita Chaudhuri;S. Bhattacharya;T. Krishnan;P. Dutta;D. Dutta;M. Bhattacharya;U. Mitra;N. Kobayashi;T. Naik
1998 ~ 2000年间，266份样本经RNA电泳检测为a组轮状病毒阳性。样本采集自加尔各答两家主要医院收治的患者。只有22%的阳性样本可以进行血清分型，其余78%无法分型。采用反转录和多重PCR技术对159份样品进行了G （VP7基因型）和P （VP4基因型）的鉴定。主要基因型为G1P[8](20%)，其次为G2P[4]（15%）和G4P[8]（6%）。在此研究期间还检测到一些不常见的基因型，G1P[4] (4%), G2P[8] (2.5%), G2P[6] (0.6%), G4P[4]（2.5%）和G4P[6]（1.25%）。22%的样本显示混合感染，38个（24%）样本的VP7或VP4都无法分型，而只有4个（2.5%）样本的两种基因都无法分型。从曼尼普尔收集的11个标本也进行了基因分型，并显示出非常高程度的基因组重组。
ABSTRACT Between 1998 and 2000, a total of 266 samples were found positive for group A rotaviruses by RNA electrophoresis. Samples were collected from patients admitted to two leading hospitals in Calcutta. Serotyping could be done only with 22% of the positive samples, leaving 78% untypeable. The G (VP7 genotypes) and P (VP4 genotypes) types were determined for 159 samples by reverse transcription and multiplex PCR. The predominant genotype was G1P[8] (20%), followed by G2P[4] (15%) and G4P[8] (6%). A number of uncommon genotypes, G1P[4] (4%), G2P[8] (2.5%), G2P[6] (0.6%), G4P[4] (2.5%), and G4P[6] (1.25%), were also detected during this study period. Twenty two percent of specimens showed mixed infections, 38 (24%) of the total samples remained untypeable for either VP7 or VP4, while only 4 (2.5%) of the samples were untypeable for both genes. Eleven specimens collected from Manipur were also genotyped and revealed a very high degree of genomic reassortment.
影响因子：
12.7
作者：
Okada, J;Urasawa, T;Urasawa, S
通讯作者：
Urasawa, S