Normalization of mass cytometry data with bead standards.
Normalization of mass cytometry data with bead standards.
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DOI：
10.1002/cyto.a.22271
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发表时间：
2013-05
期刊：
影响因子：
3.7
通讯作者：
Bendall, Sean C.
中科院分区：
文献类型：
--
作者：
Finck, Rachel;Simonds, Erin F.;Jager, Astraea;Krishnaswamy, Smita;Sachs, Karen;Fantl, Wendy;Pe'er, Dana;Nolan, Garry P.;Bendall, Sean C.
质谱流式细胞术使用原子质谱法结合同位素纯报告元件，目前可测量每个单细胞多达40个参数。与任何定量技术一样，质量保证和标准化协议是一个基本需求。在质谱流式细胞术的情况下，由于仪器性能的变化以及计划维护之间的间隔而导致的信号随时间的变化必须考虑在内，然后进行标准化。在这里，将样品与包埋有金属镧系元素的聚苯乙烯珠混合，允许在多天的数据采集中监测质谱细胞仪的性能。本文所述的方案包括在质谱流式细胞仪上同时测量微珠和细胞，随后提取基于微珠的特征，以及应用能够校正短期和长期信号波动的算法。归一化后保留的珠粒强度的变化也可用于确定数据质量。应用该算法对人外周血样本进行为期一个月的纵向分析，将中值信号波动范围从4.9倍降低到1.3倍。
Mass cytometry uses atomic mass spectrometry combined with isotopically pure reporter elements to currently measure as many as 40 parameters per single cell. As with any quantitative technology, there is a fundamental need for quality assurance and normalization protocols. In the case of mass cytometry, the signal variation over time due to changes in instrument performance combined with intervals between scheduled maintenance must be accounted for and then normalized. Here, samples were mixed with polystyrene beads embedded with metal lanthanides, allowing monitoring of mass cytometry instrument performance over multiple days of data acquisition. The protocol described here includes simultaneous measurements of beads and cells on the mass cytometer, subsequent extraction of the bead-based signature, and the application of an algorithm enabling correction of both short- and long-term signal fluctuations. The variation in the intensity of the beads that remains after normalization may also be used to determine data quality. Application of the algorithm to a one-month longitudinal analysis of a human peripheral blood sample reduced the range of median signal fluctuation from 4.9-fold to 1.3-fold.
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DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
15
作者：
Abdelrahman, Ahmed I.;Dai, Sheng;Thickett, Stuart C.;Ornatsky, Olga;Bandura, Dmitry;Baranov, Vladimir;Winnik, Mitchell A.
通讯作者：
Winnik, Mitchell A.
影响因子：
7.4
作者：
Bandura, Dmitry R.;Baranov, Vladimir I.;Tanner, Scott D.
通讯作者：
Tanner, Scott D.
影响因子：
3.4
作者：
Abdelrahman AI;Ornatsky O;Bandura D;Baranov V;Kinach R;Dai S;Thickett SC;Tanner S;Winnik MA
通讯作者：
Winnik MA
影响因子：
--
作者：
Dendrou CA;Fung E;Esposito L;Todd JA;Wicker LS;Plagnol V
通讯作者：
Plagnol V