Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.
Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.
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DOI：
10.1039/b921770c
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发表时间：
2010
影响因子：
3.4
通讯作者：
Winnik MA
中科院分区：
文献类型：
--
作者：
Abdelrahman AI;Ornatsky O;Bandura D;Baranov V;Kinach R;Dai S;Thickett SC;Tanner S;Winnik MA
我们研究了含金属的聚苯乙烯珠的质量细胞仪仪器的校准，基于电感耦合等离子体离子源和飞行时间质谱仪的单粒子分析仪的适用性。还验证了这些含金属的微珠可用作该仪器的内标物。以丙烯酸为共聚单体，采用多段分散聚合法合成了该微球。丙烯酸充当配体以将金属离子锚在珠内部。质谱细胞仪能够逐珠测量金属含量并确定金属含量分布。通过分散聚合，涉及三个阶段合成的珠被证明具有较窄的珠到珠的变化，在其镧系元素含量比珠通过2阶段分散聚合合成。在六个月的时间内，珠粒在不同pH值的水溶液中表现出不显著的镧系元素含量释放。当与KG1a或U937细胞系混合时，含金属的聚合物珠显示不影响对特异性附着于这些细胞的元素标记抗体的金属含量的质谱细胞计数响应。
We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.
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影响因子：
3.4
作者：
Baranov, VI;Quinn, ZA;Tanner, SD
通讯作者：
Tanner, SD
影响因子：
15
作者：
Song, JS;Tronc, F;Winnik, MA
通讯作者：
Winnik, MA
影响因子：
46.9
作者：
Han, MY;Gao, XH;Nie, S
通讯作者：
Nie, S
影响因子：
16.6
作者：
Lou, Xudong;Zhang, Guohua;Winnik, Mitchell A.
通讯作者：
Winnik, Mitchell A.
影响因子：
82.9
作者：
Chattopadhyay, Pratip K.;Price, David A.;Roederer, Mario
通讯作者：
Roederer, Mario