Pyrene-assisted efficient photolysis of disulfide bonds in DNA-based molecular engineering.
Pyrene-assisted efficient photolysis of disulfide bonds in DNA-based molecular engineering.
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DOI：
10.1021/am1007886
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发表时间：
2010-12
影响因子：
9.5
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
You, Mingxu;Zhu, Zhi;Liu, Haipeng;Gulbakan, Basri;Han, Da;Wang, Ruowen;Williams, Kathryn R.;Tan, Weihong
提出了一种有效的Py辅助光解二硫键的方法，在350 nm波长下，仅用0.3W的光照射20分钟，即可裂解77%的二硫键。通过使用DNA骨架，可以观察到依赖于距离的裂解途径和形成自由基的光反应机制。为了证明这种二硫化芘分子组装体的生物医学应用，进一步研究了DNA胶束结构和DNAzyme类似物。实现了对DNA胶束的快速光驱动分解，允许进一步设计在目标区域和特定时间的受控药物释放。DNAzyme类似物可以进行符合Michaelis-Menton方程的多个周转反应，kcat为10.2min-1，Km为46.3μM(0.3W 350 nm光源)，与常见的DNAzyme(如8-17 DNAzyme)相当。
An efficient pyrene-assisted method has been developed for the photolysis of disulfide bonds, with 77% of disulfides cleaved after only 20 minutes of irradiation (0.3W) at 350nm. By employing a DNA framework, it was possible to observe both a distance-dependent cleavage pathway and a radical-forming photoreaction mechanism. To demonstrate the biomedical applications of such pyrene disulfide molecular assemblies, a DNA micelle structure and DNAzyme analog were further studied. Rapid photo-driven disassembly of DNA micelles was achieved, allowing the further design of controlled pharmaceutical release at the target region and at a specific time. The DNAzyme analog can carry out multiple turnover reactions that follow the Michaelis-Menton equation, with a kcat of 10.2 min-1 and a KM of 46.3μM (0.3W 350nm light source), comparable to that of common DNAzymes, e.g., 8-17 DNAzyme.
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影响因子：
15
作者：
BENT, DV;HAYON, E
通讯作者：
HAYON, E
影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
15
作者：
Conlon, Patrick;Yang, Chaoyong James;Tan, Weihong
通讯作者：
Tan, Weihong
蛋白质二硫键异构酶 AGR2 对于肠粘液的产生至关重要
DOI：
10.1073/pnas.0808722106
发表时间：
2009-04-28
影响因子：
11.1
作者：
Park, Sung-Woo;Zhen, Guohua;Erle, David J.
通讯作者：
Erle, David J.
影响因子：
15
作者：
Yim, TJ;Liu, JW;Dordick, JS
通讯作者：
Dordick, JS