Rice ubiquitin-conjugating enzyme OsUBC26 is essential for immunity to the blast fungus Magnaporthe oryzae.
Rice ubiquitin-conjugating enzyme OsUBC26 is essential for immunity to the blast fungus Magnaporthe oryzae.
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DOI：
10.1111/mpp.13132
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发表时间：
2021-12
影响因子：
4.9
通讯作者：
Lu GD
中科院分区：
文献类型：
--
作者：
Liu X;Song L;Zhang H;Lin Y;Shen X;Guo J;Su M;Shi G;Wang Z;Lu GD
泛素结合酶(E2)在植物免疫中的作用还不是很清楚。在本研究中，水稻泛素结合酶OsUBC26具有防御稻瘟病菌的功能。米曲霉接种和茉莉酸甲酯处理诱导了OsUBC26的表达。OsUBC26的RNA干扰系和CRISPR/Cas9缺失突变体都降低了水稻对稻瘟病菌的抗性。在OsUBC26缺失突变体中，WRKY45表达下调。体外E2活性测定表明，OsUBC26是一种活性较高的泛素结合酶。以OsUBC26为诱饵的酵母双杂交实验证明，环型E3连接酶UCIP2是一种相互作用蛋白。免疫共沉淀实验证实了OsUBC26与UCIP2之间的相互作用。UCIP2的CRISPR/Cas9突变体也表现出对稻瘟病菌的折衷抗性。以UCIP2为诱饵的酵母双杂交筛选表明，APIP6是UCIP2的结合伙伴。此外，OsUBC26与稻瘟病菌的无毒效应因子APIP6泛素化AvrPiz-t和OsUBC26零突变共同作用，削弱了AvrPiz-t在水稻细胞中的蛋白酶体降解。综上所述，OsUBC26通过调节WRKY45的表达和与APIP6等E3连接酶协同作用，中和白粉菌的效应蛋白AvrPiz-t，在水稻抗病中发挥重要作用。水稻泛素结合酶OsUBC26通过调节WRKY45的表达和与APIP6协同作用促进AvrPiz-t的泛素化和降解，对稻瘟菌的免疫至关重要。
The functions of ubiquitin‐conjugating enzymes (E2) in plant immunity are not well understood. In this study, OsUBC26, a rice ubiquitin‐conjugating enzyme, was characterized in the defence against Magnaporthe oryzae. The expression of OsUBC26 was induced by M. oryzae inoculation and methyl jasmonate treatment. Both RNA interference lines and CRISPR/Cas9 null mutants of OsUBC26 reduced rice resistance to M. oryzae. WRKY45 was down‐regulated in OsUBC26 null mutants. In vitro E2 activity assay indicated that OsUBC26 is an active ubiquitin‐conjugating enzyme. Yeast two‐hybrid assays using OsUBC26 as bait identified the RING‐type E3 ligase UCIP2 as an interacting protein. Coimmunoprecipitation assays confirmed the interaction between OsUBC26 and UCIP2. The CRISPR/Cas9 mutants of UCIP2 also showed compromised resistance to M. oryzae. Yeast two‐hybrid screening using UCIP2 as bait revealed that APIP6 is a binding partner of UCIP2. Moreover, OsUBC26 working with APIP6 ubiquitinateds AvrPiz‐t, an avirulence effector of M. oryzae, and OsUBC26 null mutation impaired the proteasome degradation of AvrPiz‐t in rice cells. In summary, OsUBC26 plays important roles in rice disease resistance by regulating WRKY45 expression and working with E3 ligases such as APIP6 to counteract the effector protein AvrPiz‐t from M. oryzae. Rice ubiquitin‐conjugating enzyme OsUBC26 is essential for immunity to blast fungus by regulating WRKY45 expression and working with APIP6 to promote the ubiquitination and degradation of AvrPiz‐t.
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影响因子：
4.6
作者：
Millyard L;Lee J;Zhang C;Yates G;Sadanandom A
通讯作者：
Sadanandom A
影响因子：
4.9
作者：
Shimono, Masaki;Koga, Hironori;Takatsuji, Hiroshi
通讯作者：
Takatsuji, Hiroshi
影响因子：
27.5
作者：
Ma, Xingliang;Zhang, Qunyu;Liu, Yao-Guang
通讯作者：
Liu, Yao-Guang
E2S：结构上经济和功能上充满。
DOI：
10.1042/bj20100985
发表时间：
2011-01-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Wenzel DM;Stoll KE;Klevit RE
通讯作者：
Klevit RE
影响因子：
5.6
作者：
Stone SL
通讯作者：
Stone SL