Histological analysis of low dose NMU effects in the rat mammary gland.
Histological analysis of low dose NMU effects in the rat mammary gland.
复制标题
大鼠乳腺中低剂量NMU作用的组织学分析。
DOI：
10.1186/1471-2407-9-267
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发表时间：
2009-08-03
期刊：
影响因子：
3.8
通讯作者：
Soto AM
中科院分区：
文献类型：
--
作者：
Murray TJ;Ucci AA;Maffini MV;Sonnenschein C;Soto AM
本研究旨在探讨低剂量N-亚硝基甲基脲(NMU)染毒对雌性Wistar-Furth大鼠乳腺组织的影响。30~40只49~58日龄处女大鼠按体重单次注射10、20、30、50 mg NMU。对照组10只，仅给予0.9%氯化钠溶液。每周评估可触及的乳腺肿瘤的形成，并在治疗后12周、22周和25-30周处死动物，对所有乳腺病变和肿瘤进行全面的组织学分析。除了预测肿瘤数量随着NMU剂量的增加而增加和肿瘤潜伏期的减少外，我们还观察到在所有NMU剂量下，乳腺中的一些显微镜病变和其他上皮异常。在暴露于10或20 mg NMU/kg体重的大鼠中，观察到两种类型的非肿瘤性组织学变化：(I)腺泡结构数量增加，通常伴随着分泌物进入管腔，这通常与妊娠和哺乳有关；(Ii)上皮管管腔内脱落的上皮细胞数量增加。这项研究建立了低剂量暴露的基线，并确定了NMU暴露导致的乳腺组织学特征。此外，该系统还提供了一个理想的平台，用于评估受环境影响而受到保护或易患癌症的动物的相对易感性。
Our objective was to assess the histological changes in mammary glands of the female Wistar-Furth rat as a result of low dose exposure to N-nitrosomethylurea (NMU). Groups of 30–40 virgin female rats of between 49–58 days old received a single injection of 10, 20, 30 or 50 mg NMU/kg body weight (BW). A group of 10 control rats received 0.9% NaCl solution only. The formation of palpable mammary gland tumors was assessed weekly and, upon sacrifice at 12, 22 and 25–30 weeks after treatment, we performed a comprehensive histological analysis of all mammary gland lesions and tumors. Alongside the predicted increase in tumor number and decrease in tumor latency with increasing NMU dose, we observed a number of microscopic lesions and other epithelial abnormalities in the mammary glands for all NMU doses. Two types of non-neoplastic histological changes were observed in rats exposed to 10 or 20 mg NMU/kg BW: namely, (i) an increase in the number of acinar structures often accompanied by secretion into the lumen which is normally associated with pregnancy and lactation, and (ii) an increase in the number of epithelial cells sloughed into the lumen of the epithelial ducts. This study establishes a baseline for low-dose exposure and defines the histological features in the mammary gland resulting from NMU exposure. Furthermore, this system provides an ideal platform for evaluating the relative susceptibility of animals protected from, or predisposed to, developing cancer through environmental influences.
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影响因子：
3.3
作者：
Vandenberg, Laura N.;Maffini, Maricel V.;Schaeberle, Cheryl M.;Ucci, Angelo A.;Sonnenschein, Carlos;Rubin, Beverly S.;Soto, Ana M.
通讯作者：
Soto, Ana M.
DOI：
10.1073/pnas.91.9.3749
发表时间：
1994-04-26
影响因子：
11.1
作者：
CHA, RS;THILLY, WG;ZARBL, H
通讯作者：
ZARBL, H
影响因子：
4.8
作者：
Vandenberg, Laura N.;Maffini, Maricel V.;Soto, Ana M.
通讯作者：
Soto, Ana M.
影响因子：
2.2
作者：
Narisawa, T;Fukaura, Y
通讯作者：
Fukaura, Y
影响因子：
4.7
作者：
THOMPSON, HJ;ADLAKHA, H;SINGH, M
通讯作者：
SINGH, M