DNA dosimeter measurements of beam profile using a novel simultaneous processing technique.
DNA dosimeter measurements of beam profile using a novel simultaneous processing technique.
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DOI：
10.1016/j.apradiso.2020.109316
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Kirby N
中科院分区：
文献类型：
--
作者：
Bui B;McConnell K;Obeidat M;Saenz D;Papanikolaou N;Shim EY;Kirby N
DNA剂量计（DNAd）是以前开发的，使用双链断裂（DSB）来测量剂量。该剂量计已经过测试，可在已建立瞬态带电粒子平衡（TCPE）的情况下测量剂量。双链断裂概率（PDSBo）是剂量计中断裂双链DNA（dsDNA）与初始未断裂dsDNA的比率，用于定量DSB并与剂量相关。本工作的目标是产生一种新的技术来处理和分析DNAd和定量DNA-DSB。该技术包括同时处理多个DNAd，并建立一种新的双链断裂概率（PDSBn）形式，然后通过进行光束半影测量来测试非TCPE条件下的DNAd。该技术使用384孔板，在10 × 10 cm视野边缘进行测量，并与胶片测量结果进行比较。在这些半影测量期间，在观察距辐射场中心4.1 cm和4.55 cm处较高梯度区域的位置差异时，PDSBo与胶片的一致距离分别为0.38 cm和0.26 cm，而PDSBn与胶片的一致距离分别为0.11 cm和0.06 cm。最后，开发的新分离技术将分析25个样品所需的时间从200分钟缩短到30分钟。
A DNA dosimeter (DNAd) was previously developed that uses double-strand breaks (DSB) to measure dose. This dosimeter has been tested to measure dose in scenarios where transient-charged particle equilibrium (TCPE) has been established. The probability of double strand break (PDSBo), which is the ratio of broken double-stranded DNA (dsDNA) to the initial unbroken dsDNA in the dosimeter, was used to quantify DSBs and related to dose. The goal of this work is to produce a new technique to process and analyze the DNAd and quantify DNA-DSBs. This technique included simultaneously processing multiple DNAds and also establishing a new form to the probability of double strand break (PDSBn), which was then used to test the DNAd in a non-TCPE condition by taking beam penumbra measurements. The technique utilized a 384-well plate, and the measurements were made at the edge of a 10 × 10 cm field and compared to film measurements. During these penumbra measurements, while observing the positional differences in the higher gradient region at 4.1 and 4.55 cm from the center of the radiation field, the distance to agreement of PDSBo to film were 0.38 cm and 0.26cm while the distance to agreement of PDSBn to film were 0.11 cm and 0.06 cm, respectively. Finally, the developed new separation technique reduced the time needed for the analysis of 25 samples from 200 minutes to 30 minutes.
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DOI：
10.1016/j.mrgentox.2006.03.002
发表时间：
2006-06-16
影响因子：
1.9
作者：
Kumaravel, T. S.;Jha, Awadhesh N.
通讯作者：
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DOI：
10.1042/bj20080413
发表时间：
2009-02-01
期刊：
The Biochemical journal
影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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