DNA dosimetry assessment for sunscreen genotoxic photoprotection.
DNA dosimetry assessment for sunscreen genotoxic photoprotection.
复制标题
DOI：
10.1371/journal.pone.0040344
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Menck CF
中科院分区：
文献类型：
--
作者：
Schuch AP;Lago JC;Yagura T;Menck CF
由于在过去几十年中太阳紫外线辐射（UV）入射率的增加，防晒霜的使用已被广泛用于皮肤保护。然而，考虑到阳光诱导的DNA损伤的高效率，关键是要改进目前用于评估保护因子的方法。系统使用DNA剂量计提供了一种评价防晒霜对每日紫外线辐射诱导的DNA损伤的光保护作用的替代方法。DNA的防晒系数（DNA-SPF）是通过使用特定的DNA修复酶来计算的，它被定义为与未受保护的对照样品相比，抑制环丁烷嘧啶二聚体（CPD）和氧化DNA碱基生成的能力。最初评估了五种不同的商业品牌的防晒霜，进一步的研究扩展了分析，包括代表各种配方和防晒系数（SPF）的17种其他产品。总的来说，所有SPF 30防晒霜的商业品牌都提供了足够的保护，防止模拟阳光遗传毒性。此外，该DNA生物传感器可用于快速筛选各种防晒制剂的生物保护性能。DNA剂量计的应用被证明是一种替代的，互补的，可靠的方法，在DNA损伤的水平上量化防晒霜的光保护。
Due to the increase of solar ultraviolet radiation (UV) incidence over the last few decades, the use of sunscreen has been widely adopted for skin protection. However, considering the high efficiency of sunlight-induced DNA lesions, it is critical to improve upon the current approaches that are used to evaluate protection factors. An alternative approach to evaluate the photoprotection provided by sunscreens against daily UV radiation-induced DNA damage is provided by the systematic use of a DNA dosimeter. The Sun Protection Factor for DNA (DNA-SPF) is calculated by using specific DNA repair enzymes, and it is defined as the capacity for inhibiting the generation of cyclobutane pyrimidine dimers (CPD) and oxidised DNA bases compared with unprotected control samples. Five different commercial brands of sunscreen were initially evaluated, and further studies extended the analysis to include 17 other products representing various formulations and Sun Protection Factors (SPF). Overall, all of the commercial brands of SPF 30 sunscreens provided sufficient protection against simulated sunlight genotoxicity. In addition, this DNA biosensor was useful for rapidly screening the biological protection properties of the various sunscreen formulations. The application of the DNA dosimeter is demonstrated as an alternative, complementary, and reliable method for the quantification of sunscreen photoprotection at the level of DNA damage.
登录
查看更多内容
DOI：
10.1073/pnas.0604213103
发表时间：
2006-09-12
影响因子：
11.1
作者：
Mouret, Stephane;Baudouin, Caroline;Douki, Thierry
通讯作者：
Douki, Thierry
影响因子：
2.9
作者：
Douki, T;Reynaud-Angelin, A;Sage, E
通讯作者：
Sage, E
影响因子：
3.8
作者：
Mouret, Stephane;Charveron, Marie;Douki, Thierry
通讯作者：
Douki, Thierry
影响因子：
3.3
作者：
RIBEIRO, DT;MADZAK, C;MENCK, CFM
通讯作者：
MENCK, CFM
DOI：
10.1111/j.1600-0781.2008.00370.x
发表时间：
2008-10-01
影响因子：
2.6
作者：
Lejeune, Francois;Christiaens, Francois;Bernerd, Francois
通讯作者：
Bernerd, Francois