Engineering of glycerol utilization in Gluconobacter oxydans 621H for biocatalyst preparation in a low-cost way.
Engineering of glycerol utilization in Gluconobacter oxydans 621H for biocatalyst preparation in a low-cost way.
复制标题
氧化葡萄糖杆菌 621H 中甘油的利用工程以低成本方式制备生物催化剂
DOI：
10.1186/s12934-018-1001-0
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发表时间：
2018-10-08
影响因子：
6.4
通讯作者：
Ma C
中科院分区：
文献类型：
--
作者：
Yan J;Xu J;Cao M;Li Z;Xu C;Wang X;Yang C;Xu P;Gao C;Ma C
氧化葡糖醛酸全细胞广泛应用于各种生物催化过程。高浓度的山梨醇通常用于复杂介质中以制备生物催化剂。开发一种制备低成本底物的生物催化剂的替代方法对于工业应用具有重要意义。 氧化葡萄球菌621 H在以甘油为唯一碳源的无机盐培养基中生长，甘油是生物燃料工业中不可避免的废弃物。基于本研究阐明的甘油利用机制，主要的多元醇脱氢酶（GOX 0854）和膜结合醇脱氢酶（GOX 1068）可以竞争利用甘油，但在生物催化剂制备中没有发挥明显的作用。因此，与这两种酶相关的基因被删除。G. oxydans GOX 1068和GOX 0854可以从甘油制备，其生物量产率比G. oxydans 621 H.利用G. oxydans GOX 1068和GOX 0854作为生物催化剂，从58.4 g L-1木糖以1.05 g g g-1的产率生产61.6 g L-1木糖酸盐。该方法是高效制备G.降低成本的氧化丹。除了从木糖生产木糖酸之外，还可以利用代谢工程G.由甘油制备的氧化丹。本文的在线版本（10.1186/s12934-018-1001-0）包含补充材料，可供授权用户使用。
Whole cells of Gluconobacter oxydans are widely used in various biocatalytic processes. Sorbitol at high concentrations is commonly used in complex media to prepare biocatalysts. Exploiting an alternative process for preparation of biocatalysts with low cost substrates is of importance for industrial applications. G. oxydans 621H was confirmed to have the ability to grow in mineral salts medium with glycerol, an inevitable waste generated from industry of biofuels, as the sole carbon source. Based on the glycerol utilization mechanism elucidated in this study, the major polyol dehydrogenase (GOX0854) and the membrane-bound alcohol dehydrogenase (GOX1068) can competitively utilize glycerol but play no obvious roles in the biocatalyst preparation. Thus, the genes related to these two enzymes were deleted. Whole cells of G. oxydans ∆GOX1068∆GOX0854 can be prepared from glycerol with a 2.4-fold higher biomass yield than that of G. oxydans 621H. Using whole cells of G. oxydans ∆GOX1068∆GOX0854 as the biocatalyst, 61.6 g L−1 xylonate was produced from 58.4 g L−1 xylose at a yield of 1.05 g g−1. This process is an example of efficient preparation of whole cells of G. oxydans with reduced cost. Besides xylonate production from xylose, other biocatalytic processes might also be developed using whole cells of metabolic engineered G. oxydans prepared from glycerol. The online version of this article (10.1186/s12934-018-1001-0) contains supplementary material, which is available to authorized users.
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影响因子：
11.4
作者：
Dikshit, Pritam Kumar;Kharmawlong, Gracel Joe;Moholkar, Vijayanand S.
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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