How to kill the honey bee larva: genomic potential and virulence mechanisms of Paenibacillus larvae.
How to kill the honey bee larva: genomic potential and virulence mechanisms of Paenibacillus larvae.
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如何杀死蜜蜂幼虫：幼虫的基因组潜力和毒力机制。
DOI：
10.1371/journal.pone.0090914
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Daniel R
中科院分区：
文献类型：
--
作者：
Djukic M;Brzuszkiewicz E;Fünfhaus A;Voss J;Gollnow K;Poppinga L;Liesegang H;Garcia-Gonzalez E;Genersch E;Daniel R
Paenibacillus幼虫是一种革兰氏阳性细菌病原体，可引起蜜蜂最严重的传染病American Foulbrood （AFB）。为了研究弓形虫幼虫的基因组潜力，对两种不同基因型的2株弓形虫进行了测序并进行了比较基因组分析。遗传型为ERIC II的小蠊幼虫DSM 25430的全基因组序列为4056006 bp，包含3928个预测蛋白编码基因。基因型为ERIC I的小蠊幼虫菌株DSM 25719基因组草图序列为4,579,589 bp，包含4,868个蛋白编码基因。这两种菌株都含有9.7 kb的质粒，并编码大量毒力相关蛋白，如毒素和胶原酶。此外，还鉴定了编码产生非核糖体肽或多酮的大型多模酶的基因。在菌株DSM 25719的基因组中，鉴定并分析了7个毒素相关位点。其中五个编码了假定的功能性毒素。菌株DSM 25430的基因组中有几个毒素位点与菌株DSM 25719的基因组中相应的位点相似，但由于点突变或转座酶的破坏而失去功能。虽然这两种菌株都引起AFB，但在基因组大小、转座酶的数量和组成、插入元件、预测噬菌体区域和菌株特异性岛状区域等方面观察到显著差异。转座酶、整合酶和重组酶是基因组可塑性的重要驱动因素。在应变DSM 25430和应变DSM 25719中分别发现390和273个可移动元件。两株菌株的比较基因组学揭示了通过水平基因转移获得毒力因子，并为进化和致病性提供了见解。
Paenibacillus larvae, a Gram positive bacterial pathogen, causes American Foulbrood (AFB), which is the most serious infectious disease of honey bees. In order to investigate the genomic potential of P. larvae, two strains belonging to two different genotypes were sequenced and used for comparative genome analysis. The complete genome sequence of P. larvae strain DSM 25430 (genotype ERIC II) consisted of 4,056,006 bp and harbored 3,928 predicted protein-encoding genes. The draft genome sequence of P. larvae strain DSM 25719 (genotype ERIC I) comprised 4,579,589 bp and contained 4,868 protein-encoding genes. Both strains harbored a 9.7 kb plasmid and encoded a large number of virulence-associated proteins such as toxins and collagenases. In addition, genes encoding large multimodular enzymes producing nonribosomally peptides or polyketides were identified. In the genome of strain DSM 25719 seven toxin associated loci were identified and analyzed. Five of them encoded putatively functional toxins. The genome of strain DSM 25430 harbored several toxin loci that showed similarity to corresponding loci in the genome of strain DSM 25719, but were non-functional due to point mutations or disruption by transposases. Although both strains cause AFB, significant differences between the genomes were observed including genome size, number and composition of transposases, insertion elements, predicted phage regions, and strain-specific island-like regions. Transposases, integrases and recombinases are important drivers for genome plasticity. A total of 390 and 273 mobile elements were found in strain DSM 25430 and strain DSM 25719, respectively. Comparative genomics of both strains revealed acquisition of virulence factors by horizontal gene transfer and provided insights into evolution and pathogenicity.
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影响因子：
14.9
作者：
Ansari, MZ;Yadav, G;Mohanty, D
通讯作者：
Mohanty, D
影响因子：
1.9
作者：
Alippi, AM;Reynaldi, FJ;Aguilar, OM
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影响因子：
3.4
作者：
Antunez, Karina;Anido, Matilde;Zunino, Pablo
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影响因子：
3.3
作者：
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通讯作者：
Genersch, Elke
DOI：
10.1073/pnas.96.23.13294
发表时间：
1999-11-09
影响因子：
11.1
作者：
Duitman, EH;Hamoen, LW;Vater, J
通讯作者：
Vater, J