Fast and Sensitive Measurement of Specific Antigen-Antibody Binding Reactions With Magnetic Nanoparticles and HTS SQUID
Fast and Sensitive Measurement of Specific Antigen-Antibody Binding Reactions With Magnetic Nanoparticles and HTS SQUID
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使用磁性纳米颗粒和 HTS SQUID 快速灵敏地测量特异性抗原抗体结合反应
DOI：
10.1109/tasc.2009.2019246
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发表时间：
2009
影响因子：
1.8
通讯作者：
Dag Winkler
中科院分区：
文献类型：
--
作者：
Fredrik Öisjöen;J. Schneiderman;Magdalena Zaborowska;Karthikeyan Shunmugavel;Per Magnelind;Alexey Kalaboukhov;Karolina Petersson;Andrea Prieto Astalan;Christer Johansson;Dag Winkler
我们开发了一套高温超导SQUID系统，用于测量包覆有抗体的液体悬浮磁性纳米颗粒(MNPs)的布朗弛豫磁信号。基线为3 mm的平面YBa2Cu3O7-x DC SQUID梯度仪允许测量系统在非屏蔽环境中稳定运行。用我们的系统表征了前列腺特异性抗原-抗体结合复合体诱导的MNPs的聚集，并以Imego AB使用感应线圈磁强计进行的频域测量为基准。我们估计我们的免疫分析分析物灵敏度上限为1.8毫微克/根赫兹。
We have developed an HTS SQUID system for both time and frequency domain measurements of magnetic signals from the Brownian relaxation of liquid-suspended magnetic nanoparticles (MNPs) coated with antibodies. A planar YBa2Cu3O7-x dc SQUID gradiometer with a baseline of 3 mm allows stable operation of the measurement system in an unshielded environment. Agglomeration of MNPs induced by prostate specific antigen-antibody binding complexes is characterized with our system and benchmarked with frequency domain measurements performed by Imego AB using an induction coil magnetometer. We estimate an upper bound for our immunoassay analyte sensitivity of 1.8 ng/radicHz.
用于生物免疫分析的磁标记和高T_e超导量子干涉装置
DOI：
--
发表时间：
2005
期刊：
IEICE Trans. Electron. E88-C, no.2
影响因子：
--
作者：
小林;田中;中本;森泉;上山;W.S.Yerazunis;K.Enpuku et al.
通讯作者：
K.Enpuku et al.
影响因子：
1.8
作者：
Tsukamoto, A;Saitoh, K;Enpuku, K
通讯作者：
Enpuku, K