Current development in genetic engineering strategies of Bacillus species.

Current development in genetic engineering strategies of Bacillus species.
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芽孢杆菌属基因工程策略的最新进展

DOI:
10.1186/1475-2859-13-63
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发表时间:
2014-05-03
影响因子:
6.4
通讯作者:
Zhang D
Zhang D
中科院分区:
工程技术2区
文献类型:
--
作者:
Dong H;Zhang D

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对工业上重要的芽孢杆菌基因组的完整测序和注释加深了我们对它们的性质的了解,并使其他芽孢杆菌物种的遗传操作取得了进展。后基因组研究需要简单而高效的工具来实现基因操作。在这里,我们总结了芽孢杆菌基因工程策略的最新进展。我们回顾了在芽孢杆菌物种中开发的可用的遗传工具,以及在其他物种中开发的可能也适用于芽孢杆菌的方法。此外,我们解决了现有方法的局限性和挑战,并讨论了未来的研究前景,开发新的和有用的工具,以芽孢杆菌物种的基因改造。
The complete sequencing and annotation of the genomes of industrially-important Bacillus species has enhanced our understanding of their properties, and allowed advances in genetic manipulations in other Bacillus species. Post-genomic studies require simple and highly efficient tools to enable genetic manipulation. Here, we summarize the recent progress in genetic engineering strategies for Bacillus species. We review the available genetic tools that have been developed in Bacillus species, as well as methods developed in other species that may also be applicable in Bacillus. Furthermore, we address the limitations and challenges of the existing methods, and discuss the future research prospects in developing novel and useful tools for genetic modification of Bacillus species.
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