Induction of complement-dependent and -independent neutralizing antibodies by recombinant-derived human cytomegalovirus gp55-116 (gB)
Induction of complement-dependent and -independent neutralizing antibodies by recombinant-derived human cytomegalovirus gp55-116 (gB)
复制标题
重组衍生的人巨细胞病毒 gp55-116 (gB) 诱导补体依赖性和非依赖性中和抗体
作者：
W. Britt;L. Vugler;E. Stephens
人巨细胞病毒（HCMV）包膜糖蛋白复合物gp55-116在大肠杆菌和重组痘苗病毒感染的细胞中均有表达。大肠杆菌产生的单个蛋白的Mr为100,000，其大小接近hcmv感染细胞中发现的非糖基化gp55-116前体的大小。被重组痘苗病毒感染的细胞含有三种细胞内Mr 16万、15万和55万，通过与gp55反应的单克隆抗体检测。这些重组蛋白的免疫学特性比较表明，几种HCMV gp55-116单克隆抗体和HCMV感染患者的血清与牛痘病毒衍生蛋白发生反应，而一组更有限的单克隆抗体识别大肠杆菌产生的蛋白。用大肠杆菌或痘苗病毒重组HCMV gp55-116免疫小鼠可产生病毒中和抗体。与重组痘苗病毒免疫后几乎只产生补体依赖性中和抗体相反，大肠杆菌衍生蛋白诱导了补体非依赖性中和抗体。
The human cytomegalovirus (HCMV) envelope glycoprotein complex gp55-116 was expressed in both Escherichia coli and cells infected with a recombinant vaccinia virus. E. coli produced a single protein of Mr 100,000 which approximated the size of the nonglycosylated gp55-116 precursor found in HCMV-infected cells. Cells infected with the recombinant vaccinia virus contained three intracellular forms of Mr 160,000, 150,000, and 55,000 which were detected by a monoclonal antibody reactive with gp55. Comparison of the immunological properties of these recombinant proteins indicated that several of the HCMV gp55-116 monoclonal antibodies and sera from patients infected with HCMV reacted with the vaccinia virus-derived proteins whereas a more restricted group of monoclonal antibodies recognized the E. coli-produced protein. Immunization of mice with either E. coli or vaccinia virus recombinant HCMV gp55-116 resulted in production of virus-neutralizing antibodies. In contrast to the almost exclusive production of complement-dependent neutralizing antibodies following immunization with recombinant vaccinia virus, the E. coli-derived protein induced complement-independent neutralizing antibodies.
人巨细胞病毒多肽在体内刺激中和抗体。
影响因子：
3.7
作者：
Rasmussen,L;Mullenax,J;Nelson,M;Merigan,TC
通讯作者：
Merigan,TC
水泡性口炎病毒糖蛋白基因在大肠杆菌中的条件表达。
DOI：
10.1073/pnas.78.11.6670
发表时间：
1981
影响因子：
11.1
作者：
Rose,JK;Shafferman,A
通讯作者：
Shafferman,A