Molecular clone and functional study of a novel hepatoma associated gene.
Molecular clone and functional study of a novel hepatoma associated gene.
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新型肝癌相关基因的分子克隆及功能研究。
DOI：
10.3892/ijo.2013.1766
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发表时间：
2013-03
影响因子：
5.2
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Zhou G;Xie P;Li G;Li Y
HTA是我们前期通过生物信息学策略筛选到的一个新的肝癌相关基因。本研究采用3 '端RACE和5'端RACE技术，克隆到了HTA基因全长序列，全长1414 bp，开放阅读框（ORF）由3个外显子和2个内含子组成。HTA的mRNA有两种剪接类型。北方杂交结果显示，1.4 kb HTA mRNA和1.7 kb HTA mRNA在肝癌细胞系HepG 2和QGY-7703中有表达，而在正常细胞系L02和人脐静脉内皮细胞中无表达。稳定转染HTA基因在肝细胞系QSG-7701中过表达，可提高其增殖速度和集落形成能力，改变细胞周期分布。这些结果表明，HTA基因是一个潜在的治疗肝癌的目标，其基因结构和序列信息的澄清，为未来的研究提供了必要的工具。
HTA is a novel hepatoma associated gene screened by bioinformatic strategies in our previous study. In the present investigation, the full-length sequence of the HTA gene was cloned by 3'-rapid amplification of cDNA ends (RACE) and 5'-RACE, which was 1414 bp and the open reading frame (ORF) was constituted with 3 exons and 2 introns. There are two types of splicing of the mRNA of HTA. Northern blot analysis showed that the 1.4 kb HTA mRNA and 1.7 kb HTA mRNA transcripts were present in the hepatocellular carcinoma (HCC) cell lines HepG2 and QGY-7703 but not in the normal cell line L02 and the human umbilical vein endothelial cells (HUVECs). Overexpression of the HTA gene in the hepatic cell line QSG-7701 via stable transfection can promote its proliferation rate and colony forming ability and change the cell cycle distribution of the cell lines. These results showed that the HTA gene is a potential therapeutic target in HCC and the clarification of its gene structure and sequence information provide an essential tool for future research.
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DOI：
10.1007/s00432-010-0767-1
发表时间：
2010-08-01
影响因子：
3.6
作者：
Liu, Yan;Li, Yuehui;Li, Guancheng
通讯作者：
Li, Guancheng
影响因子：
3.5
作者：
Kelemen O;Convertini P;Zhang Z;Wen Y;Shen M;Falaleeva M;Stamm S
通讯作者：
Stamm S
影响因子：
10.5
作者：
Villanueva A;Minguez B;Forner A;Reig M;Llovet JM
通讯作者：
Llovet JM
影响因子：
3.5
作者：
D. Pratyush;Shalbha Tiwari;Ashok Kumar;S. Singh
通讯作者：
D. Pratyush;Shalbha Tiwari;Ashok Kumar;S. Singh
DOI：
--
发表时间：
2011-05
期刊：
Missouri medicine
影响因子：
--
作者：
B. DuBray;W. Chapman;C. Anderson
通讯作者：
B. DuBray;W. Chapman;C. Anderson