Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing.
Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing.
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DOI：
10.1186/1471-2180-8-43
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发表时间：
2008-03-06
期刊：
影响因子：
4.2
通讯作者：
Wolcott, Randall D.
中科院分区：
文献类型：
--
作者：
Dowd, Scot E.;Sun, Yan;Secor, Patrick R.;Rhoads, Daniel D.;Wolcott, Benjamin M.;James, Garth A.;Wolcott, Randall D.
慢性伤口致病性生物膜是宿主 - 病原体环境，多种细菌在此定殖并共存。这些细菌群体相互协作以促进自身生存以及感染的慢性化。很少有研究对不同类型慢性伤口生物膜内的细菌群体进行广泛调查。利用基于16S的3种独立分子扩增技术，随后进行焦磷酸测序、鸟枪法桑格测序和变性梯度凝胶电泳，对三种慢性伤口类型（糖尿病足溃疡（D）、下肢静脉性溃疡（V）和压疮（P））致病性生物膜中的主要细菌群体进行了调查。
在所有慢性伤口类型的生物膜中都存在特定的主要细菌群体，包括葡萄球菌属、假单胞菌属、消化链球菌属、肠杆菌属、嗜麦芽窄食单胞菌属、芬戈尔德菌属和沙雷氏菌属等。每种伤口类型的细菌群体都存在显著差异，例如在压疮中，62%的细菌群体被鉴定为专性厌氧菌。还存在一些已被鉴定但不被认为是伤口病原体的细菌群体，如毗邻贫养菌和红假单胞菌属等。分子分析结果也与传统基于培养的诊断方法所获结果进行了比较。只有一种伤口类型的培养方法正确鉴定出了主要细菌群体，这表明需要改进诊断方法。
如果临床医生能够更好地了解伤口的微生物群，他们将对伤口的生态有更深入的理解，并能够更好地管理伤口愈合，改善患者的预后。这项研究强调了有必要开始将慢性伤口致病性生物膜作为多物种实体进行评估、研究和治疗，以改善患者的治疗效果。这项调查还将促进新型分子诊断工具的开创和开发，这些工具可用于鉴定慢性伤口致病性生物膜以及其他医用生物膜感染的群落组成。
Chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. These bacterial populations cooperate to promote their own survival and the chronic nature of the infection. Few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. The use of 3 separate16S-based molecular amplifications followed by pyrosequencing, shotgun Sanger sequencing, and denaturing gradient gel electrophoresis were utilized to survey the major populations of bacteria that occur in the pathogenic biofilms of three types of chronic wound types: diabetic foot ulcers (D), venous leg ulcers (V), and pressure ulcers (P). There are specific major populations of bacteria that were evident in the biofilms of all chronic wound types, including Staphylococcus, Pseudomonas, Peptoniphilus, Enterobacter, Stenotrophomonas, Finegoldia, and Serratia spp. Each of the wound types reveals marked differences in bacterial populations, such as pressure ulcers in which 62% of the populations were identified as obligate anaerobes. There were also populations of bacteria that were identified but not recognized as wound pathogens, such as Abiotrophia para-adiacens and Rhodopseudomonas spp. Results of molecular analyses were also compared to those obtained using traditional culture-based diagnostics. Only in one wound type did culture methods correctly identify the primary bacterial population indicating the need for improved diagnostic methods. If clinicians can gain a better understanding of the wound's microbiota, it will give them a greater understanding of the wound's ecology and will allow them to better manage healing of the wound improving the prognosis of patients. This research highlights the necessity to begin evaluating, studying, and treating chronic wound pathogenic biofilms as multi-species entities in order to improve the outcomes of patients. This survey will also foster the pioneering and development of new molecular diagnostic tools, which can be used to identify the community compositions of chronic wound pathogenic biofilms and other medical biofilm infections.
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影响因子：
0.9
作者：
Bauer, Stephen M;Bauer, Richard J;Velazquez, Omaida C
通讯作者：
Velazquez, Omaida C
影响因子：
3
作者：
Brem, H;Kirsner, RS;Falanga, V
通讯作者：
Falanga, V
影响因子：
3
作者：
BURTON, CS
通讯作者：
BURTON, CS
DOI：
10.1097/01.ta.0000240175.18189.af
发表时间：
2007-07-01
影响因子：
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作者：
Armour, Alexis D.;Shankowsky, Heather A.;Tredget, Edward E.
通讯作者：
Tredget, Edward E.
DOI：
10.1097/01.blo.0000175128.44966.d9
发表时间：
2005-08-01
影响因子：
4.2
作者：
Costerton, JW
通讯作者：
Costerton, JW