Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide
Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide
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真菌竞争因子的表征：分生孢子细胞壁相关抗真菌肽的产生
DOI：
10.1371/journal.ppat.1008518
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发表时间：
2020-04
期刊：
影响因子：
--
通讯作者：
Yanhua Fan
中科院分区：
文献类型：
--
作者：
Sheng Tong;Maolian Li;Nemat O. Keyhani;Yu Liu;Min Yuan;Dongmei Lin;Dan Jin;Xianbi Li;Yan Pei;Yanhua Fan
竞争是自然选择的基本驱动力之一。球孢白僵菌是一种土壤和植物叶面/根真菌，能够寄生昆虫宿主。土壤和植物环境通常富含B抵抗的其他真菌。巴西纳为生存而竞争。在这里，我们报告了一种抗真菌肽（BbAFP 1），特异性表达和定位于分生孢子细胞壁，并释放到周围的微环境中抑制竞争真菌的生长。B。在共培养实验中，表达BbAFP 1的球孢菌株，包括过表达菌株，抑制了芸苔链格孢的生长，而BbAFP 1的靶向基因缺失显著降低了这种抑制作用（25%）。重组BbAFP 1表现出几丁质和葡聚糖结合能力，并通过破坏膜完整性和引发活性氧（ROS）产生来抑制广泛的植物病原真菌的生长。苯丙氨酸残基（F50）有助于几丁质结合和抗真菌活性，但后者不是必需的。BbAFP 1在番茄中的表达导致转基因植株对植物真菌病原菌的抗性增强。这些结果突出了真菌竞争在形成原始竞争策略中的重要性，其中可以嵌入孢子细胞壁中的抗微生物化合物在萌发的关键初始阶段释放到环境中，以在其环境生态位中成功生长。此外，这些肽可用于增加植物对真菌病原体的抗性。
Competition is one of the fundamental driving forces of natural selection. Beauveria bassiana is a soil and plant phylloplane/root fungus capable of parasitizing insect hosts. Soil and plant environments are often enriched with other fungi against which B. bassiana competes for survival. Here, we report an antifungal peptide (BbAFP1), specifically expressed and localized to the conidial cell wall and is released into the surrounding microenvironment inhibiting growth of competing fungi. B. bassiana strains expressing BbAFP1, including overexpression strains, inhibited growth of Alternaria brassicae in co-cultured experiments, whereas targeted gene deletion of BbAFP1 significantly decreased (25%) this inhibitory effect. Recombinant BbAFP1 showed chitin and glucan binding abilities, and growth inhibition of a wide range of phytopathogenic fungi by disrupting membrane integrity and eliciting reactive oxygen species (ROS) production. A phenylalanine residue (F50) contributes to chitin binding and antifungal activity, but was not required for the latter. Expression of BbAFP1 in tomato resulted in transgenic plants with enhanced resistance to plant fungal pathogens. These results highlight the importance of fungal competition in shaping primitive competition strategies, with antimicrobial compounds that can be embedded in the spore cell wall to be released into the environment during the critical initial phases of germination for successful growth in its environmental niche. Furthermore, these peptides can be exploited to increase plant resistance to fungal pathogens.
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影响因子：
3.1
作者：
Hegedues, Nikoletta;Sigl, Claudia;Zadra, Ivo;Pocsi, Istvan;Marx, Florentine
通讯作者：
Marx, Florentine
细胞分裂素通过破坏 PIN3a 介导的胚珠表皮中生长素的不对称积累来抑制棉纤维的起始
影响因子：
6.9
作者：
Zeng Jianyan;Zhang Mi;Hou Lei;Bai Wenqin;Yan Xingying;Hou Nan;Wang Hongxing;Huang Juan;Zhao Juan;Pei Yan
通讯作者：
Pei Yan
影响因子：
4.5
作者：
Meyer V;Jung S
通讯作者：
Jung S
影响因子：
4.3
作者：
Fizil, Adam;Gaspari, Zoltan;Barna, Terezia;Marx, Florentine;Batta, Gyula
通讯作者：
Batta, Gyula
影响因子：
4.6
作者：
Garrigues S;Gandía M;Popa C;Borics A;Marx F;Coca M;Marcos JF;Manzanares P
通讯作者：
Manzanares P