Resuscitation of viable but non-culturable bacteria to enhance the cellulose-degrading capability of bacterial community in composting.
Resuscitation of viable but non-culturable bacteria to enhance the cellulose-degrading capability of bacterial community in composting.
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复活活的但不可培养的细菌以增强堆肥中细菌群落的纤维素降解能力
DOI：
10.1111/1751-7915.13256
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发表时间：
2018-05
影响因子：
5.7
通讯作者：
Sun F
中科院分区：
文献类型：
--
作者：
Su X;Zhang S;Mei R;Zhang Y;Hashmi MZ;Liu J;Lin H;Ding L;Sun F
目前，我们对分离的纤维素分解菌的了解大多来自传统的平板分离技术。然而，由于进入活的但不可培养（VBNC）状态，许多细菌物种的可培养性受到复苏促进因子（Rpfs）的控制。因此，在本研究中，将来自藤黄微球菌的Rpf添加到培养基中，以评估其在细菌分离中的作用以及对堆肥中细菌群落的纤维素降解能力的增强作用。结果表明，变形菌和放线菌是堆肥样品中的两个主要门。Rpf的引入可以分离出一些独特的细菌物种。用Rpf处理和不用Rpf处理的富集培养物的纤维素酶活性表明，Rpf处理显著增强纤维素酶活性。在Rpf添加中独特的10个分离物显示羧甲基纤维素酶（CMCase）活性，而6个分离物具有滤纸纤维素酶（FPCase）活性。这项研究为更广泛的纤维素降解剂提供了新的见解，可用于加强纤维素废物处理。
Nowadays, much of what we know regarding the isolated cellulolytic bacteria comes from the conventional plate separation techniques. However, the culturability of many bacterial species is controlled by resuscitation‐promoting factors (Rpfs) due to entering a viable but non‐culturable (VBNC) state. Therefore, in this study, Rpf from Micrococcus luteus was added in the culture medium to evaluate its role in bacterial isolation and enhanced effects on cellulose‐degrading capability of bacterial community in the compost. It was found that Proteobacteria and Actinobacteria were two main phyla in the compost sample. The introduction of Rpf could isolate some unique bacterial species. The cellulase activity of enrichment cultures with and without Rpf treatment revealed that Rpf treatment significantly enhanced cellulase activity. Ten isolates unique in Rpf addition displayed carboxymethyl‐cellulase (CMCase) activity, while six isolates possessed filter paper cellulase (FPCase) activity. This study provides new insights into broader cellulose degraders, which could be utilized for enhancing cellulosic waste treatment.
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