Circular RNAs expressed in chorionic villi are probably involved in the occurrence of recurrent spontaneous abortion
Circular RNAs expressed in chorionic villi are probably involved in the occurrence of recurrent spontaneous abortion
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绒毛膜绒毛中表达的环状RNA可能与复发性流产的发生有关
DOI：
10.1016/j.biopha.2017.01.172
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发表时间：
2017-04
影响因子：
7.5
通讯作者：
项目申请人）
中科院分区：
文献类型：
--
作者：
Qian Yating;Wang Xin;Ruan Hongjie;Rui Can;Mao Pengyuan;Cheng Qing;Jia Ruizhe（贾瑞喆;项目申请人）
复发性自然流产（RSA）是困扰世界各地妇女的一种病因不明的常见疾病。近年来，关于环状RNA（circular RNA，circRNA）在妇产科领域的研究不断涌现。本研究的目的是研究RSA患者与相应的健康孕妇绒毛中circRNA的表达，并推断这些circRNA在RSA发生中的潜在作用。我们选择了35对RSA患者和健康对照受试者，并使用circRNA微阵列分析了每组3对绒毛样品中的异常circRNA表达。共检测到335条上调和259条下调的circRNA，594条异常表达的circRNA，（FC ≥ 2.0，P ≤ 0.05），考虑到各circRNA的原始含量、倍数变化差异以及不同引物的限制性，有4个circRNA表达下调使用实时定量逆转录-聚合酶链反应（qRT-PCR），选择4个表达上调的基因（hsa_circRNA_104948、104547、101319和104938）和4个表达上调的基因（hsa_circRNA_102116、100709、102424和103102）作为验证基因。还建立了配对检验和受试者工作特征（ROC）曲线。含量验证结果与芯片结果完全一致（所有验证circRNA均P ≤ 0.05）。这8种circRNA的ROC曲线下面积（AUC）均大于0.5。通过论文比较对RSA患者中的circRNA进行了进一步的功能分析。总之，RSA中绒毛circRNA的异常表达可能通过circRNA的miRNA海绵功能在其发病中起重要作用。深入分析详细的机制将是值得的。
Recurrent spontaneous abortion (RSA) is an unknown aetiology common disease troubled women all over the world. Recent studies of circular RNAs (circRNAs) in gynecology and obstetrics area have sprung up. Our purpose in this study was to investigate the expression of circRNAs in chorionic villi from patients with RSA versus corresponding healthy gravidas, and to infer the potential role of these circRNAs in RSA occurrence. We selected 35 pairs of RSA patients and healthy control subjects, and analyzed the aberrant circRNA expression in three pairs of villi samples from each group using circRNA microarray. Altogether, including 335 up-regulated and 259 down-regulated circRNAs, 594 aberrant expressed circRNAs (FC ≥ 2.0, P ≤ 0.05) were found. Considering the raw content of each circRNAs, fold change differences and the restriction of the divergent primers, four down-regulated circRNAs (hsa_circRNA_104948, 104547, 101319 and 104938) and four up-regulated (hsa_circRNA_102116, 100709, 102424 and 103102) were selected as validation genes, using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Pairedt-test and receiver operating characteristic (ROC) curves were also established. The result of content verification was accordant exactly with the microarray chip outcome (P ≤ 0.05 in all validation circRNAs). The area under the ROC curves (AUCs) of these eight circRNAs were all greater than 0.5. Further function analysis of circRNAs in RSA patients was performed by paper comparison. Taken together, aberrant villi circRNA expression in RSA played a vital role in its nosogenesis possibly via miRNA sponge function of circRNAs. Deeper analysis of the detailed mechanisms will be worthwhile.
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