Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model
Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model
复制标题
C-吡喃木糖苷衍生物对体外 3D 口腔粘膜模型上皮再生的影响
DOI：
10.1080/09168451.2016.1153957
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
K. Izumi
中科院分区：
文献类型：
--
作者：
A. Uenoyama;I. Kakizaki;A. Shiomi;N. Saito;Y. Hara;Taro Saito;H. Ohnuki;H. Kato;R. Takagi;T. Maeda;K. Izumi
在临床使用前识别上皮较差的不合格组织工程口腔粘膜移植物对于确保再生医学的质量保证/控制至关重要，从而导致移植成功。本研究探讨了一种C-吡喃木糖苷衍生物β-D-吡喃木糖苷-正丙烷-2-酮（XPP）对口腔上皮再生的影响。使用三维口腔粘膜模型，我们分析了上皮结构、糖胺聚糖（GAG）合成、基底膜区标志物表达水平和Akt/mTOR信号传导底物的变化。与对照组相比，2 mM XPP处理增加了上皮的平均厚度和最小厚度，并减少了上皮厚度的变化。随着GAG含量和/或组成的改变，其刺激decorin和syndecan-1的表达，并促进整合素α6、CD 44和Akt/mTOR信号底物的表达。这些发现表明，XPP补充有助于一致的上皮再生。此外，这些标记物的上调可能在提高口腔粘膜上皮的质量中起作用。图形摘要XPP对体外3D口腔粘膜模型中一致的上皮再生有影响（A：组织学检查，B：组织形态学分析）。
Identifying substandard tissue-engineered oral mucosa grafts with a poor epithelium before clinical use is critical to ensure quality assurance/control in regenerative medicine, leading to success of grafting. This study investigated the effects of one of the C-xylopyranoside derivatives, β-D-xylopyranoside-n-propane-2-one (XPP), on oral epithelial regeneration. Using a three-dimensional oral mucosa model, we analyzed changes of the epithelial structure, glycosaminoglycan (GAG) synthesis, the expression levels of basement membrane zone markers, and substrates of Akt/mTOR signaling. Compared with the control, 2 mM XPP treatment increased the mean and minimal epithelial thickness, and reduced the variation of epithelial thickness. It also stimulated expressions of decorin and syndecan-1 with change of GAG amount and/or composition, and enhanced the expressions of integrin α6, CD44, and Akt/mTOR signaling substrates. These findings suggest that XPP supplementation contributes to consistent epithelial regeneration. Moreover, upregulation of those markers may play a role in increasing the quality of the oral mucosal epithelium. Graphical abstract XPP had an effect on consistent epithelial regeneration in an in vitro 3D oral mucosa model (A: Histologic examination, B: Histomorphometric analysis).
DOI：
10.11607/jomi.te11
发表时间：
2013-09
期刊：
The International journal of oral & maxillofacial implants
影响因子：
--
作者：
Izumi K;Neiva RF;Feinberg SE
通讯作者：
Feinberg SE
监管基于细胞的再生医学：未来的挑战。
影响因子：
2.7
作者：
Ali RR
通讯作者：
Ali RR