Intraoral grafting of tissue-engineered human oral mucosa.
Intraoral grafting of tissue-engineered human oral mucosa.
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DOI：
10.11607/jomi.te11
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Feinberg SE
中科院分区：
文献类型：
--
作者：
Izumi K;Neiva RF;Feinberg SE
本研究的主要目的是评价组织工程化人离体口腔粘膜等效物（EVPOME）在口内移植手术中的安全性。次要目的是评估移植的EVPOME在产生角化粘膜表面上皮中的功效。五名患者符合入选标准，有一个粘膜牙龈缺陷或缺乏角化牙龈的非磨牙，沿着与足够的牙间骨高度的放射学证据，招募作为受试者，以增加角化牙龈的宽度在缺陷部位。取硬腭的穿刺活检标本以获得口腔角质形成细胞，将其扩增、接种并培养在无细胞真皮基质上以用于制造EVPOME。将EVPOME移植物直接应用于完整的骨膜床并固定到位。在基线（活检标本回收）和术后7、14、30、90和180天，记录每例受试者的菌斑指数和牙龈指数。此外，在基线、30、90和180天记录探诊深度、角化牙龈宽度和角化牙龈厚度。研究期间，在任何受试者中均未观察到EVPOME的并发症或不良反应。角化牙龈宽度平均增加3 mm（范围3 - 4 mm）。角化牙龈厚度的平均增益为1 mm（范围，1至2 mm）。未观察到探测深度的显著变化。基于这些发现，可以得出结论，EVPOME用于口内是安全的，并且能够增加牙齿周围的角化组织。未来的临床试验需要进一步探索这种潜力。
The primary objective of this study was to evaluate the safety of a tissue-engineered human ex vivo–produced oral mucosa equivalent (EVPOME) in intraoral grafting procedures. The secondary objective was to assess the efficacy of the grafted EVPOME in producing a keratinized mucosal surface epithelium. Five patients who met the inclusion criteria of having one mucogingival defect or a lack of keratinized gingiva on a nonmolar tooth, along with radiographic evidence of sufficient interdental bone height, were recruited as subjects to increase the width of keratinized gingiva at the defect site. A punch biopsy specimen of the hard palate was taken to acquire oral keratinocytes, which were expanded, seeded, and cultured on an acellular dermal matrix for fabrication of an EVPOME. EVPOME grafts were applied directly over an intact periosteal bed and secured in place. At baseline (biopsy specimen retrieval) and at 7, 14, 30, 90, and 180 days postsurgery, Plaque Index and Gingival Index were recorded for each subject. In addition, probing depths, keratinized gingival width, and keratinized gingival thickness were recorded at baseline, 30, 90, and 180 days. No complications or adverse reactions to EVPOME were observed in any subjects during the study. The mean gain in keratinized gingival width was 3 mm (range, 3 to 4 mm). The mean gain in keratinized gingival thickness was 1 mm (range, 1 to 2 mm). No significant changes in probing depths were observed. Based on these findings, it can be concluded that EVPOME is safe for intraoral use and has the ability to augment keratinized tissue around teeth. Future clinical trials are needed to further explore this potential.
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影响因子：
2.7
作者：
Izumi, K;Song, JH;Feinberg, SE
通讯作者：
Feinberg, SE
影响因子：
7.6
作者：
Izumi, K;Terashi, H;Feinberg, SE
通讯作者：
Feinberg, SE
DOI：
10.1054/ijom.2002.0365
发表时间：
2003-04-01
影响因子：
2.4
作者：
Izumi, K;Feinberg, SE;Yoshizawa, M
通讯作者：
Yoshizawa, M
影响因子：
1.9
作者：
Lauer, G;Schimming, R
通讯作者：
Schimming, R
影响因子：
3
作者：
HILL, MW;BERG, JH;MACKENZIE, IC
通讯作者：
MACKENZIE, IC