Treat the "Untreatable" by a Photothermal Agent: Triggering Heat and Immunological Responses for Rabies Virus Inactivation.
Treat the "Untreatable" by a Photothermal Agent: Triggering Heat and Immunological Responses for Rabies Virus Inactivation.
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用光热剂治疗“无法治疗的疾病”：触发热和免疫反应以灭活狂犬病病毒
DOI：
10.1002/advs.202205461
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发表时间：
2023-01
期刊：
影响因子：
15.1
通讯作者：
Wang, Hualei
中科院分区：
文献类型：
--
作者：
Bai, Yujie;Huang, Pei;Feng, Na;Li, Yuanyuan;Huang, Jingbo;Jin, Hongli;Zhang, Mengyao;Sun, Jingxuan;Li, Nan;Zhang, Haili;Xia, Xianzhu;Tang, Ben Zhong;Wang, Hualei
狂犬病是由狂犬病毒（RABV）引起的一种致命的神经系统人畜共患疾病，在医疗系统不完善的地区，仍然无法获得已批准的暴露后预防（PEP）程序。尽管已经提出了PEP和感染后治疗（PIT）的策略，但由于治疗程序的复杂性和治疗效果有限，制定有效的治疗策略仍然是狂犬病研究的圣杯。本文提出一种结合光热疗法（PTT）和光热触发免疫效应的简便方法，同时实现有效的PEP和PIT。所设计的光热剂（N+TT - mCB纳米颗粒）具有正电荷官能团和高光热效率，有利于病毒靶向和灭活。经原位PTT治疗后，小鼠感染部位的病毒水平显著降低，病毒向大脑的转移明显受到抑制。此外，通过颅内注射N+TT‐mCB和激光照射，感染后3天小鼠的存活率提高。本研究为RABV的有效治疗提供了平台，为今后的抗病毒研究开辟了新的途径。设计了一种基于光热剂的治疗无法治愈的狂犬病毒的简便方法，该方法涉及高光热效率和带正电的官能团。该病毒通过光热灭活和光热触发免疫效应，可同时实现有效的暴露后预防和感染后治疗。
Rabies is a fatal neurological zoonotic disease caused by the rabies virus (RABV), and the approved post‐exposure prophylaxis (PEP) procedure remains unavailable in areas with inadequate medical systems. Although strategies have been proposed for PEP and postinfection treatment (PIT), because of the complexity of the treatment procedures and the limited curative outcome, developing an effective treatment strategy remains a holy grail in rabies research. Herein, a facile approach is proposed involving photothermal therapy (PTT) and photothermally triggered immunological effects to realize effective PEP and PIT simultaneously. The designed photothermal agent (N+TT‐mCB nanoparticles) featured positively charged functional groups and high photo‐to‐heat efficiency, which are favorable for virus targeting and inactivation. The level of the virus at the site of infection in mice is significantly decreased upon treatment with orthotopic PTT, and the transfer of the virus to the brain is significantly inhibited. Furthermore, the survival ratio of the mice three days postinfection is increased by intracranial injection of N+TT‐mCB and laser irradiation. Overall, this work provides a platform for the effective treatment of RABV and opens a new avenue for future antiviral studies. A facile approach that treats the untreatable rabies virus based on a photothermal agent is designed involving high photo‐to‐heat efficiency and positively charged functional groups. The virus is inactivated by photothermal therapy and photothermally triggered immunological effects which can realize effective post‐exposure prophylaxis and postinfection treatment simultaneously.
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DOI：
10.1038/nrrheum.2015.169
发表时间：
2016-01
期刊：
Nature reviews. Rheumatology
影响因子：
--
作者：
Kalliolias GD;Ivashkiv LB
通讯作者：
Ivashkiv LB
DOI：
10.1038/nri2851
发表时间：
2010-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5
作者：
Gupta PK;Sonwane AA;Singh NK;Meshram CD;Dahiya SS;Pawar SS;Gupta SP;Chaturvedi VK;Saini M
通讯作者：
Saini M
影响因子：
5.4
作者：
Chai, Qingqing;He, Wen Q.;Fu, Zhen F.
通讯作者：
Fu, Zhen F.
影响因子：
5.4
作者：
Faber, M;Bette, M;Weihe, E
通讯作者：
Weihe, E