Long non-coding RNAs (lncRNAs) NEAT1 and MALAT1 are differentially expressed in severe COVID-19 patients: An integrated single-cell analysis.
Long non-coding RNAs (lncRNAs) NEAT1 and MALAT1 are differentially expressed in severe COVID-19 patients: An integrated single-cell analysis.
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DOI：
10.1371/journal.pone.0261242
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Perkins DL
中科院分区：
文献类型：
--
作者：
Huang K;Wang C;Vagts C;Raguveer V;Finn PW;Perkins DL
过度活跃和破坏性炎症是严重而非轻度2019冠状病毒病（COVID-19）的标志。为了揭示严重和轻度COVID-19之间的关键炎症差异，我们应用了无偏的单细胞转录组学分析。我们整合了两个单细胞RNA-seq数据集与COVID-19患者样本，一个对支气管肺泡灌洗（BAL）细胞进行测序，另一个对外周血单核细胞（PBMC）进行测序。然后分析合并的细胞群，重点是与疾病严重程度相关的基因。免疫调节性长链非编码RNA（lncRNAs）NEAT 1和MALAT 1在多种细胞类型的轻度和重度患者之间高度差异表达。在这些相同的细胞类型中，同时检测到参与细胞应激反应和凋亡调节的其他严重性相关基因表明，这些lncRNA的促炎功能可能会促进细胞应激和损伤。因此，NEAT 1和MALAT 1是COVID-19免疫失调的潜在组分，可为严重程度相关的诊断措施或治疗提供靶点。
Hyperactive and damaging inflammation is a hallmark of severe rather than mild Coronavirus disease 2019 (COVID-19). To uncover key inflammatory differentiators between severe and mild COVID-19, we applied an unbiased single-cell transcriptomic analysis. We integrated two single-cell RNA-seq datasets with COVID-19 patient samples, one that sequenced bronchoalveolar lavage (BAL) cells and one that sequenced peripheral blood mononuclear cells (PBMCs). The combined cell population was then analyzed with a focus on genes associated with disease severity. The immunomodulatory long non-coding RNAs (lncRNAs) NEAT1 and MALAT1 were highly differentially expressed between mild and severe patients in multiple cell types. Within those same cell types, the concurrent detection of other severity-associated genes involved in cellular stress response and apoptosis regulation suggests that the pro-inflammatory functions of these lncRNAs may foster cell stress and damage. Thus, NEAT1 and MALAT1 are potential components of immune dysregulation in COVID-19 that may provide targets for severity related diagnostic measures or therapy.
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