Distinct Features and Functions of Systemic and Mucosal Humoral Immunity Among SARS-CoV-2 Convalescent Individuals.
Distinct Features and Functions of Systemic and Mucosal Humoral Immunity Among SARS-CoV-2 Convalescent Individuals.
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DOI：
10.3389/fimmu.2020.618685
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发表时间：
2020
影响因子：
7.3
通讯作者：
Ackerman ME
中科院分区：
文献类型：
--
作者：
Butler SE;Crowley AR;Natarajan H;Xu S;Weiner JA;Bobak CA;Mattox DE;Lee J;Wieland-Alter W;Connor RI;Wright PF;Ackerman ME
了解SARS-CoV-2感染的体液免疫反应将在疫苗和基于抗体的干预措施的开发中发挥关键作用。我们报告了经历了不同严重程度疾病的恢复期个体的全身和粘膜抗体反应。尽管中和和抗体介导的效应子功能的评估揭示了血清中的多功能抗体应答，但在鼻洗液样品中仅稳健的中和和吞噬作用是明显的。血清中和和效应功能与全身SARS-CoV-2特异性IgG反应强度相关，而粘膜中和与鼻SARS-CoV-2特异性伊加相关。抗体耗竭实验支持这些相关性的机制相关性。鼻腔伊加反应、粘膜病毒中和和较轻疾病之间的相关性表明在较大的自然感染队列中评估粘膜免疫的重要性。在入口处的抗体反应的进一步表征可以定义其有助于保护免受感染或降低住院风险的能力，为公共卫生评估策略和疫苗开发工作提供信息。
Understanding humoral immune responses to SARS-CoV-2 infection will play a critical role in the development of vaccines and antibody-based interventions. We report systemic and mucosal antibody responses in convalescent individuals who experienced varying severity of disease. Whereas assessment of neutralization and antibody-mediated effector functions revealed polyfunctional antibody responses in serum, only robust neutralization and phagocytosis were apparent in nasal wash samples. Serum neutralization and effector functions correlated with systemic SARS-CoV-2-specific IgG response magnitude, while mucosal neutralization was associated with nasal SARS-CoV-2-specific IgA. Antibody depletion experiments support the mechanistic relevance of these correlations. Associations between nasal IgA responses, virus neutralization at the mucosa, and less severe disease suggest the importance of assessing mucosal immunity in larger natural infection cohorts. Further characterization of antibody responses at the portal of entry may define their ability to contribute to protection from infection or reduced risk of hospitalization, informing public health assessment strategies and vaccine development efforts.
影响因子：
1.8
作者：
Saif LJ
通讯作者：
Saif LJ