Development of nasal, fecal and serum isotype-specific antibodies in calves challenged with bovine coronavirus or rotavirus.
Development of nasal, fecal and serum isotype-specific antibodies in calves challenged with bovine coronavirus or rotavirus.
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DOI：
10.1016/0165-2427(87)90159-0
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发表时间：
1987-12
影响因子：
1.8
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Saif LJ
未哺乳的无特定病原体小牛在3-4周龄时接种牛冠状病毒，鼻内（IN）或口服（O），或O + IN（O/IN）或O接种牛轮状病毒。用免疫荧光染色（IF）、ELISA或免疫电镜（IEM）检测鼻或粪便样本中的病毒脱落和病毒感染的鼻上皮细胞。通过ELISA测定血清、鼻和粪便样品中的同种型特异性抗体滴度。犊牛接种冠状病毒后，粪便中可检出病毒，鼻上皮细胞中可检出病毒。IN接种小牛的鼻散毒持续时间长于O接种小牛，并且IN和O接种小牛的鼻散毒持续时间长于粪便散毒。所有小牛均发生腹泻，但无呼吸道疾病体征。接种轮状病毒的小牛有类似的腹泻和粪便脱落模式，但通常比接种冠状病毒的小牛持续时间短。未检测到轮状病毒鼻脱落。在大多数小牛中，在暴露后7-10天（DPE）的粪便和鼻分泌物中检测到IgM抗体反应峰值，先于10-14 DPE时出现的伊加反应峰值。即使在轮状病毒接种小牛中没有病毒经鼻散毒的情况下，所有病毒接种小牛均发生鼻抗体应答。接种冠状病毒的小牛在粪便和鼻腔样本中的IgM和伊加抗体滴度高于接种轮状病毒的小牛。在大多数接种小牛中，IgM或伊加抗体的最大滴度与粪便或鼻病毒脱落的停止相关。IgM和伊加抗体出现在血清中的类似序列，但伊加抗体持续时间较短，比粪便或鼻腔样本。血清IgG 1抗体应答通常先于IgG 2应答，并且在14-21 DPE后在大多数小牛中占主导地位。
Unsuckled specific pathogen free calves were inoculated at 3–4 weeks of age, either intranasally (IN) or orally (O) with bovine coronavirus or O plus IN (O/IN) or O with bovine rotavirus. Shedding of virus in nasal or fecal samples, and virus-infected nasal epithelial cells were detected using immunofluorescent staining (IF), ELISA or immune electron microscopy (IEM). Isotype-specific antibody titers in sera, nasal and fecal samples were determined by ELISA. Calves inoculated with coronavirus shed virus in feces and virus was detected in nasal epithelial cells. Nasal shedding persisted longer in IN-inoculated calves than in O-inoculated calves and longer than fecal shedding in both IN and O-inoculated calves. Diarrhea occurred in all calves, but there were no signs of respiratory disease. Calves inoculated with rotavirus had similar patterns of diarrhea and fecal shedding, but generally of shorter duration than in coronavirus-inoculated calves. No nasal shedding of rotavirus was detected. Peak IgM antibody responess, in most calves, were detected in fecal and nasal speciments at 7–10 days post-exposure (DPE), preceeding peak IgA responses which occurred at 10–14 DPE. The nasal antibody responses occurred in all virus-inoculated calves even in the absence of nasal shedding of virus in rotavirus-inoculated calves. Calves inoculated with coronavirus had higher titers of IgM and IgA antibodies in fecal and nasal samples than rotavirus-inoculated calves. In most inoculated calves, maximal titers of IgM or IgA antibodies correlated with the cessation of fecal or nasal virus shedding. A similar sequence of appearance of IgM and IgA antibodies occurred in serum, but IgA antibodies persisted for a shorter period than in fecal or nasal samples. Serum IgG1 antibody responses generally preceeded IgG2 responses and were predominant in most calves after 14–21 DPE.
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影响因子：
3.3
作者：
MCNULTY, MS;BRYSON, DG;LOGAN, EF
通讯作者：
LOGAN, EF
影响因子：
9.4
作者：
BOHL, EH;SAIF, LJ;CROSS, RF
通讯作者：
CROSS, RF
影响因子：
5.2
作者：
LEWIS, HM;PARRY, JV;VALMAN, HB
通讯作者：
VALMAN, HB
影响因子：
6.4
作者：
MCINTOSH, K;CHAO, RK;MUFSON, MA
通讯作者：
MUFSON, MA
DOI：
10.1001/archpedi.1978.02120340029004
发表时间：
1978-01-01
影响因子：
--
作者：
HIEBER, JP;SHELTON, S;MOHS, E
通讯作者：
MOHS, E