Increased expression and DNA-binding activity of transcription factor Sp1 in doxorubicin-resistant HL-60 leukemia cells
Increased expression and DNA-binding activity of transcription factor Sp1 in doxorubicin-resistant HL-60 leukemia cells
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多柔比星耐药 HL-60 白血病细胞中转录因子 Sp1 的表达和 DNA 结合活性增加
DOI：
10.1128/mcb.10.10.5541-5547.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
R. Glazer
中科院分区：
文献类型：
--
作者：
F. Borellini;A. Aquino;S. Josephs;R. Glazer
The processes responsible for the multidrug-resistant (Mdr) phenotype in Adriamycin (doxorubicin)-resistant HL-60 leukemia cells (HL-60/AR) are not defined. Since enhanced transcription of resistance-related proteins is associated with Mdr cells, we sought to determine whether changes in the expression of specific transcription factors were a feature characteristic of the Mdr process. Nuclear extracts were prepared from wild-type and resistant cells and compared for their ability to bind DNA consensus sequences for the transcription factors Sp1 and NF kappa B contained in the 5' long terminal repeat region of human immunodeficiency virus type 1. Southwestern (DNA-protein) blots showed a family of DNA-binding proteins of 105 kilodaltons (kDa) that were present only in HL-60/AR cells. Competitive gel shift assays indicated that these factors were related to transcription factor Sp1, and immunoblotting with an Sp1 antibody identified this factor as Sp1. DNase footprinting of the promoter region in the human immunodeficiency virus type 1 5' long terminal repeat showed that protection occurred at two Sp1 sites as well as two NF kappa B sites and the trans-acting region with nuclear extracts only from resistant cells. Preliminary evidence also suggests that phosphorylation may play a negative regulatory role in the activity of Sp1, since calf intestine alkaline phosphatase stimulated the DNA-binding activity of Sp1 in vitro. These results indicate that HL-60/AR cells contain an abundance of DNA-binding proteins, particularly Sp1, which probably interact with other cis-acting regulatory proteins in a cooperative manner.
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DOI：
10.1073/pnas.86.6.1865
发表时间：
1989
影响因子：
11.1
作者：
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作者：
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影响因子：
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作者：
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DOI：
10.1016/0006-291x(86)91047-8
发表时间：
1986
影响因子：
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作者：
Shoji,M;Girard,PR;Mazzei,GJ;Vogler,WR;Kuo,JF
通讯作者：
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影响因子：
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作者：
Girard,PR;Stevens,VL;Blackshear,PJ;MerrillJr,AH;Wood,JG;Kuo,JF
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