Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca).
Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca).
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针叶树R2R3-MYB转录因子：白云杉（Picea Glauca）的木制组织中的序列分析和基因表达。
DOI：
10.1186/1471-2229-7-17
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发表时间：
2007-03-30
影响因子：
5.3
通讯作者：
Mackay, John
中科院分区：
文献类型：
--
作者：
Bedon, Frank;Grima-Pettenati, Jacqueline;Mackay, John
R2 R3-MYB转录因子家族的几个成员在被子植物和裸子植物的木材形成过程中作为木质素和苯丙素代谢的调节剂。被子植物拟南芥有超过一百个R2 R3-MYBs基因，然而，在裸子植物中只发现了少数成员。我们从裸子植物白色云杉（Picea glauca）和火炬松（Pinus taeda L.）中分离并鉴定了编码R2 R3-MYB基因的全长cDNA。(five序列）。序列相似性和系统发育分析将云杉和松树序列置于大型R2 R3-MYB家族的不同亚组中，尽管其中几个序列紧密地聚集在一起。我们搜索了高度可变的C-末端区域的不同植物MYBs的保守氨基酸序列，并确定了20个图案的云杉MYBs，其中9个以前没有报道，其中3个是特定的针叶树。云杉MYB基因内含子的数目和长度差异显著，但其位置相对于被子植物MYB基因保守。定量RTPCR的MYB基因转录本丰度在根和茎组织中揭示了不同的表达模式，三个MYB基因优先表达在次生木质部，而其他人优先表达在韧皮部或无处不在。在木质部中表达的MYB基因和其他三个基因在诱导后76小时内在倾斜树木的压缩木材中上调。我们对18个针叶树R2 R3-MYB基因的调查清楚地显示了与拟南芥相似的基因家族结构。其中三个序列可能在裸子植物的木质素代谢和/或木材形成中发挥作用，包括火炬松PtMYB 4的密切同源物，其显示出调节转基因烟草中的木质素生物合成。
Several members of the R2R3-MYB family of transcription factors act as regulators of lignin and phenylpropanoid metabolism during wood formation in angiosperm and gymnosperm plants. The angiosperm Arabidopsis has over one hundred R2R3-MYBs genes; however, only a few members of this family have been discovered in gymnosperms. We isolated and characterised full-length cDNAs encoding R2R3-MYB genes from the gymnosperms white spruce, Picea glauca (13 sequences), and loblolly pine, Pinus taeda L. (five sequences). Sequence similarities and phylogenetic analyses placed the spruce and pine sequences in diverse subgroups of the large R2R3-MYB family, although several of the sequences clustered closely together. We searched the highly variable C-terminal region of diverse plant MYBs for conserved amino acid sequences and identified 20 motifs in the spruce MYBs, nine of which have not previously been reported and three of which are specific to conifers. The number and length of the introns in spruce MYB genes varied significantly, but their positions were well conserved relative to angiosperm MYB genes. Quantitative RTPCR of MYB genes transcript abundance in root and stem tissues revealed diverse expression patterns; three MYB genes were preferentially expressed in secondary xylem, whereas others were preferentially expressed in phloem or were ubiquitous. The MYB genes expressed in xylem, and three others, were up-regulated in the compression wood of leaning trees within 76 hours of induction. Our survey of 18 conifer R2R3-MYB genes clearly showed a gene family structure similar to that of Arabidopsis. Three of the sequences are likely to play a role in lignin metabolism and/or wood formation in gymnosperm trees, including a close homolog of the loblolly pine PtMYB4, shown to regulate lignin biosynthesis in transgenic tobacco.
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影响因子：
3.4
作者：
Gion, JM;Lalanne, C;Plomion, C
通讯作者：
Plomion, C
影响因子：
7.2
作者：
Gómez-Maldonado, J;Avila, C;Campbell, MM
通讯作者：
Campbell, MM
影响因子：
7.2
作者：
Newman, LJ;Perazza, DE;Campbell, MM
通讯作者：
Campbell, MM
DOI：
10.1073/pnas.1132171100
发表时间：
2003-06-10
影响因子：
11.1
作者：
Kirst, M;Johnson, AF;Sederoff, R
通讯作者：
Sederoff, R
影响因子：
7.2
作者：
Goicoechea, M;Lacombe, E;Grima-Pettenati, J
通讯作者：
Grima-Pettenati, J