The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication.
The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication.
复制标题
DOI：
10.1111/j.1365-2958.2009.06877.x
复制
发表时间：
2009-10
影响因子：
3.6
通讯作者：
Bates D
中科院分区：
文献类型：
--
作者：
Stepankiw N;Kaidow A;Boye E;Bates D
复制起始是所有生物体细胞周期中的关键事件，而大肠杆菌中的复制起点oriC是这一过程的原型模型。oriC功能所需的最小序列最初完全由使用克隆的起始片段的质粒研究确定，所述克隆的起始片段先前已显示与染色体的序列要求显著不同。使用体内重组工程策略将野生型oriCs交换为突变的oriCs，而不管它们是否是功能性起源，我们已经确定了将支持染色体复制的最小起源序列。oriC的几乎整个右半部分可以被删除，而不会失去原始功能，这需要重新评估现有的启动模型。携带新的DnaA盒缺失的163 bp最小oriC的细胞在缓慢生长条件下几乎没有表现出适应性损失，但对丰富培养基敏感，这表明作为原核起源标志的引发剂结合位点的密集包装可能已经进化到支持多叉复制增加的需求。
Replication initiation is a key event in the cell cycle of all organisms and oriC, the replication origin in Escherichia coli, serves as the prototypical model for this process. The minimal sequence required for oriC function was originally determined entirely from plasmid studies using cloned origin fragments, which have previously been shown to differ dramatically in sequence requirement from the chromosome. Using an in vivo recombineering strategy to exchange wt oriCs for mutated ones regardless of whether they are functional origins or not, we have determined the minimal origin sequence that will support chromosome replication. Nearly the entire right half of oriC could be deleted without loss of origin function, demanding a reassessment of existing models for initiation. Cells carrying the new DnaA box-depleted 163 bp minimal oriC exhibited little or no loss of fitness under slow-growth conditions, but were sensitive to rich medium, suggesting that the dense packing of initiator binding sites that is a hallmark of prokaryotic origins, has likely evolved to support the increased demands of multi-forked replication.
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DOI：
10.1073/pnas.78.12.7370
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FULLER, RS;KAGUNI, JM;KORNBERG, A
通讯作者：
KORNBERG, A
影响因子：
10.5
作者：
Fujimitsu, Kazuyuki;Senriuchi, Takayuki;Katayama, Tsutomu
通讯作者：
Katayama, Tsutomu
DOI：
10.1007/bf00334827
发表时间：
1983-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
FILUTOWICZ, M;JONCZYK, P
通讯作者：
JONCZYK, P
影响因子：
3.2
作者：
Blinkova, A;Hermandson, MJ;Walker, JR
通讯作者：
Walker, JR
影响因子：
11.4
作者：
CASSLER, MR;GRIMWADE, JE;LEONARD, AC
通讯作者：
LEONARD, AC