Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle.
Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle.
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DOI：
10.1016/j.xpro.2022.101593
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发表时间：
2022-09-16
期刊：
影响因子：
--
通讯作者：
Uezumi, Akiyoshi
中科院分区：
文献类型：
--
作者：
Kurosawa, Tamaki;Ikemoto-Uezumi, Madoka;Kaneshige, Akihiro;Fukada, So-ichiro;Uezumi, Akiyoshi
我们最近证明，间充质祖细胞在调节卫星细胞依赖性肌纤维增生在超负荷诱导的肌肉肥大过程中起着关键作用。在这里，我们描述了详细的协议，在小鼠跖肌间充质祖细胞的整装免疫荧光染色。Z-堆叠图像重建提供了全细胞图像，并且能够检查由过载诱导的间充质祖细胞中的雅普核转位。有关本方案使用和执行的完整详细信息，请参阅。将小鼠跖肌固定在硅橡胶板上进行整体染色用带硅胶垫片的盖玻片将染色的肌肉夹在中间进行z-stack成像通过z-stack图像重建进行间充质祖细胞的全细胞成像检查由过载诱导的间充质祖细胞中的雅普核易位出版者注释：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。我们最近证明，间充质祖细胞在调节卫星细胞依赖性肌纤维增生在超负荷诱导的肌肉肥大过程中起着关键作用。在这里，我们描述了详细的协议，在小鼠跖肌间充质祖细胞的整装免疫荧光染色。Z-堆叠图像重建提供了全细胞图像，并且能够检查由过载诱导的间充质祖细胞中的雅普核转位。
We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image reconstruction provides a whole-cell image and enables examination of YAP nuclear translocation in mesenchymal progenitors induced by overload. For complete details on the use and execution of this protocol, please refer to. Fixation of mouse plantaris muscle on a silicone rubber plate for whole-mount staining Sandwiching stained muscle by cover glasses with silicone spacer for z-stack imaging Whole-cell imaging of mesenchymal progenitors by z-stack image reconstruction Examination of YAP nuclear translocation in mesenchymal progenitors induced by overload Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image reconstruction provides a whole-cell image and enables examination of YAP nuclear translocation in mesenchymal progenitors induced by overload.
影响因子：
--
作者：
Kaneshige, Akihiro;Kaji, Takayuki;Saito, Hayato;Higashimoto, Tatsuyoshi;Nakamura, Ayasa;Kurosawa, Tamaki;Ikemoto-Uezumi, Madoka;Uezumi, Akiyoshi;Fukada, So-ichiro
通讯作者：
Fukada, So-ichiro
影响因子：
21.3
作者：
Uezumi, Akiyoshi;Fukada, So-ichiro;Tsuchida, Kunihiro
通讯作者：
Tsuchida, Kunihiro
影响因子：
23.9
作者：
Kaneshige, Akihiro;Kaji, Takayuki;Fukada, So-ichiro
通讯作者：
Fukada, So-ichiro