Resource partitioning of phytoplankton metabolites that support bacterial heterotrophy.
Resource partitioning of phytoplankton metabolites that support bacterial heterotrophy.
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DOI：
10.1038/s41396-020-00811-y
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Moran MA
中科院分区：
文献类型：
--
作者：
Ferrer-González FX;Widner B;Holderman NR;Glushka J;Edison AS;Kujawinski EB;Moran MA
可以预见，聚集在海洋浮游微植物周围的细菌群落主要由红杆菌门、黄杆菌门和γ变形菌门中的科组成。然而，这种一致的生态模式是否反映了基于资源的生态位分配或资源竞争的结果，需要更好地了解海洋表面微生物自养生物和异养生物之间的代谢物。我们利用两种策略对三种异养细菌与海洋硅藻共培养的摄取分子进行了表征，这两种策略通过细菌活性测定来审查外代谢产物池的生物学相关性：诊断基因的表达和外代谢产物的净减少，后者通过质谱和核磁共振检测，使用新的样品制备方法。在超过36个有细菌摄取证据的有机分子中，53%含有氮（包括核苷和氨基酸），11%是有机硫化合物（包括二羟基丙磺酸盐和二甲基磺酰丙酸盐），28%是多糖的成分（包括青虫胺素、几丁质和海藻酸盐）。在没有竞争的情况下，细菌对浮游植物衍生代谢物的利用重叠程度很低，预计这三种细菌只会利用鸟苷、脯氨酸和n-乙酰-d-氨基葡萄糖。外代谢物的吸收模式指出了海洋细菌群落转化近期光合产物的生态资源分配的关键作用。
The communities of bacteria that assemble around marine microphytoplankton are predictably dominated by Rhodobacterales, Flavobacteriales, and families within the Gammaproteobacteria. Yet whether this consistent ecological pattern reflects the result of resource-based niche partitioning or resource competition requires better knowledge of the metabolites linking microbial autotrophs and heterotrophs in the surface ocean. We characterized molecules targeted for uptake by three heterotrophic bacteria individually co-cultured with a marine diatom using two strategies that vetted the exometabolite pool for biological relevance by means of bacterial activity assays: expression of diagnostic genes and net drawdown of exometabolites, the latter detected with mass spectrometry and nuclear magnetic resonance using novel sample preparation approaches. Of the more than 36 organic molecules with evidence of bacterial uptake, 53% contained nitrogen (including nucleosides and amino acids), 11% were organic sulfur compounds (including dihydroxypropanesulfonate and dimethysulfoniopropionate), and 28% were components of polysaccharides (including chrysolaminarin, chitin, and alginate). Overlap in phytoplankton-derived metabolite use by bacteria in the absence of competition was low, and only guanosine, proline, and N-acetyl-d-glucosamine were predicted to be used by all three. Exometabolite uptake pattern points to a key role for ecological resource partitioning in the assembly marine bacterial communities transforming recent photosynthate.
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影响因子：
2.5
作者：
COLE, JJ;FINDLAY, S;PACE, ML
通讯作者：
PACE, ML
影响因子：
56.9
作者：
Azam, F
通讯作者：
Azam, F
影响因子：
2.8
作者：
Brinkkötter, A;Shakeri-Garakani, A;Lengeler, JW
通讯作者：
Lengeler, JW
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
海洋细菌-浮游植物模型系统中的识别级联和代谢物转移
影响因子：
5.1
作者：
Durham, Bryndan P.;Dearth, Stephen P.;Moran, Mary Ann
通讯作者：
Moran, Mary Ann