Possible role of hsa-miR-194-5p, via regulation of HS3ST2, in the pathogenesis of atopic dermatitis in children
Possible role of hsa-miR-194-5p, via regulation of HS3ST2, in the pathogenesis of atopic dermatitis in children
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hsa-miR-194-5p 通过调节 HS3ST2 在儿童特应性皮炎发病机制中的可能作用
DOI：
10.1684/ejd.2019.3676
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发表时间：
2019-11
影响因子：
2.5
通讯作者：
杨连娟
中科院分区：
文献类型：
--
作者：
孟丽;李民;高志琴;任弘瑾;陈健;刘小萍;蔡晴;江龙;任香玉;陈佳;杨连娟
特应性皮炎（AD）是一种常见的炎症性皮肤病。大量证据表明，非编码RNA在AD的进展中起重要作用，然而，血浆微小RNA在AD中的功能却鲜为人知。为了识别关键的血浆微小……
Atopic dermatitis (AD) is a prevalent inflammatory skin disease. Ample evidence has shown that non-coding RNAs play important roles in the progression of AD, however, the function of plasma microRNAs in AD is poorly understood. To identify key plasma microRNAs and explore their potential roles in AD. Plasma microRNAs from five children with AD and five control children were sequenced by microRNA sequencing (miRNA-seq) and five differentially expressed microRNAs were verified by RT-qPCR in 30 AD and 15 control children. The most differentially expressed microRNA, hsa-miR-194-5p, was selected for further analysis. Human epidermal keratinocytes were subjected to RNA sequencing following over-expression of hsa-miR-194-5p, and down-regulated genes were detected by RT-qPCR. The diagnostic potential of hsa-miR-194-5p in AD was evaluated based on receiver operating characteristic (ROC) curve analysis. Further hsa-miR-194-5p-regulated expression and gene-hsa-miR-194-5p interactions were evaluated by western blotting and luciferase assays, respectively. We identified 40 differentially expressed microRNAs, 26 up-regulated and 14 down-regulated, in children with AD compared with controls. Among the five verified plasma microRNAs, the most significant change was down-regulated hsa-miR-194-5p, which was shown to potentially serve as a biomarker for AD based on ROC analysis. Twenty-two down-regulated genes were observed following hsa-miR-194-5p over-expression, which were significantly associated with keratinocyte differentiation and establishment of the skin barrier. Moreover, HS3ST2 protein expression was down-regulated following over-expression of hsa-miR-194-5p, and the 3′-UTR of HS3ST2 was shown to bind to hsa-miR-194-5p. Hsa-miR-194-5p might be involved in the pathogenesis of AD by regulating HS3ST2 expression.
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影响因子：
14.2
作者：
Lu, Thomas X.;Rothenberg, Marc E.
通讯作者：
Rothenberg, Marc E.
DOI：
10.1016/j.jaci.2010.12.1124
发表时间：
2011-04
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Suárez-Fariñas M;Tintle SJ;Shemer A;Chiricozzi A;Nograles K;Cardinale I;Duan S;Bowcock AM;Krueger JG;Guttman-Yassky E
通讯作者：
Guttman-Yassky E
影响因子：
3.6
作者：
Buggiani, Gionata;Ricceri, Federica;Lotti, Torello
通讯作者：
Lotti, Torello
影响因子：
2.9
作者：
Wu, Tsung-Han;Pan, Chieh-Yu;Chen, Jyh-Yih
通讯作者：
Chen, Jyh-Yih
影响因子：
168.9
作者：
Rudikoff, D;Lebwohl, M
通讯作者：
Lebwohl, M