Dances with Membranes: Breakthroughs from Super-resolution Imaging.
Dances with Membranes: Breakthroughs from Super-resolution Imaging.
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DOI：
10.1016/bs.ctm.2015.03.008
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发表时间：
2015
影响因子：
--
通讯作者：
Hess ST
中科院分区：
文献类型：
--
作者：
Curthoys NM;Parent M;Mlodzianoski M;Nelson AJ;Lilieholm J;Butler MB;Valles M;Hess ST
生物膜组织介导了许多细胞功能，也与大量的人类疾病有关。然而，直到最近，实验方法一直无法直接可视化生物膜的纳米级细节，特别是在完整的活细胞中。已经提出了许多解释膜组织的模型，但测试这些模型需要间接的方法;直接成像活细胞膜中的蛋白质和脂质的愿望是技术进步的强烈动机。超分辨率显微镜的发展为在单个蛋白质和脂质水平上定量膜组织提供了强有力的工具，其中许多工具与活细胞兼容。以前无法解决的问题现在正在解决，膜生物学领域正在迅速发展。本章讨论了如何超分辨率显微镜的发展导致了生物膜组织领域的根本性进展。我们总结了历史和一些模型解释蛋白质是如何组织在细胞膜上，并给出了各种超分辨率技术和方法的概述超分辨率数据的量化。我们讨论了超分辨率技术在膜生物学中的应用，并特别提到肌动蛋白和肌动蛋白结合蛋白，病毒感染，线粒体，免疫细胞生物学和磷酸肌醇信号转导领域。最后，我们提出了我们的希望和期望的超分辨率显微镜在膜生物学领域的未来。
Biological membrane organization mediates numerous cellular functions and has also been connected with an immense number of human diseases. However, until recently, experimental methodologies have been unable to directly visualize the nanoscale details of biological membranes, particularly in intact living cells. Numerous models explaining membrane organization have been proposed, but testing those models has required indirect methods; the desire to directly image proteins and lipids in living cell membranes is a strong motivation for the advancement of technology. The development of super-resolution microscopy has provided powerful tools for quantification of membrane organization at the level of individual proteins and lipids, and many of these tools are compatible with living cells. Previously inaccessible questions are now being addressed, and the field of membrane biology is developing rapidly. This chapter discusses how the development of super-resolution microscopy has led to fundamental advances in the field of biological membrane organization. We summarize the history and some models explaining how proteins are organized in cell membranes, and give an overview of various super-resolution techniques and methods of quantifying super-resolution data. We discuss the application of super-resolution techniques to membrane biology in general, and also with specific reference to the fields of actin and actin-binding proteins, virus infection, mitochondria, immune cell biology, and phosphoinositide signaling. Finally, we present our hopes and expectations for the future of super-resolution microscopy in the field of membrane biology.
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影响因子：
56.9
作者：
Bates, Mark;Huang, Bo;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
DOI：
10.1124/jpet.112.192138
发表时间：
2012-09-01
影响因子：
3.5
作者：
Johri, Ashu;Beal, M. Flint
通讯作者：
Beal, M. Flint
影响因子：
3.7
作者：
Avilov, Sergiy;Berardozzi, Romain;Bourgeois, Dominique
通讯作者：
Bourgeois, Dominique
影响因子：
3.4
作者：
Bentz, J
通讯作者：
Bentz, J
影响因子：
3.4
作者：
Babcock, HP;Chen, C;Zhuang, XW
通讯作者：
Zhuang, XW