Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy.
Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy.
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DOI：
10.1007/s00125-010-1809-6
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发表时间：
2010-10
期刊：
影响因子：
8.2
通讯作者：
Butler, P. C.
中科院分区：
文献类型：
--
作者：
Butler, A. E.;Cao-Minh, L.;Galasso, R.;Rizza, R. A.;Corradin, A.;Cobelli, C.;Butler, P. C.
我们试图建立人类妊娠中β细胞数量适应性变化的程度和基础。胰腺是在尸检中从怀孕期间死亡(n = 18)、产后死亡(n = 6)或死亡时或死亡前不久未怀孕的妇女(对照组；n = 20)获得的。检测胰岛β细胞面积分数、胰岛大小和胰岛比例、胰岛细胞复制(Ki67)和细胞凋亡(TUNEL)，以及胰岛细胞新生的间接指标(胰管内胰岛素阳性细胞和胰腺内散在分布的β细胞)。在人类妊娠期间，∼使胰腺部分β细胞面积增加了1.4%，而平均β细胞大小没有变化。在怀孕期间，有更多的小胰岛，而不是胰岛大小或每个胰岛的β细胞的增加。未发现β细胞复制增加或β细胞凋亡的改变，但胰岛素阳性的导管细胞和散在的β细胞随着妊娠的增加而增加。在人类怀孕期间，β细胞数量的适应性增加并不像大多数啮齿动物报告中那样大。在人类中，这种增加是通过明显新生的小胰岛中β细胞数量的增加，而不是现有胰岛中β细胞的复制实现的，这是啮齿类动物怀孕的特征。本文的在线版本(doi：10.1007/s00125.0101809-6)包含补充材料，可供授权用户使用。
We sought to establish the extent and basis for adaptive changes in beta cell numbers in human pregnancy. Pancreas was obtained at autopsy from women who had died while pregnant (n = 18), post-partum (n = 6) or were not pregnant at or shortly before death (controls; n = 20). Pancreases were evaluated for fractional pancreatic beta cell area, islet size and islet fraction of beta cells, beta cell replication (Ki67) and apoptosis (TUNEL), and indirect markers of beta cell neogenesis (insulin-positive cells in ducts and scattered beta cells in pancreas). The pancreatic fractional beta cell area was increased by ∼1.4-fold in human pregnancy, with no change in mean beta cell size. In pregnancy there were more small islets rather than an increase in islet size or beta cells per islet. No increase in beta cell replication or change in beta cell apoptosis was detected, but duct cells positive for insulin and scattered beta cells were increased with pregnancy. The adaptive increase in beta cell numbers in human pregnancy is not as great as in most reports in rodents. This increase in humans is achieved by increased numbers of beta cells in apparently new small islets, rather than duplication of beta cells in existing islets, which is characteristic of pregnancy in rodents. The online version of this article (doi:10.1007/s00125-010-1809-6) contains supplementary material, which is available to authorised users.
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