Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: I. morphological maturation and kinetic changes of energy metabolism, albumin synthesis, and activities of some enzymes
Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: I. morphological maturation and kinetic changes of energy metabolism, albumin synthesis, and activities of some enzymes
复制标题
培养中大鼠肝球体形成和维持过程中的生化和功能变化：一、形态成熟和能量代谢、白蛋白合成和某些酶活性的动力学变化
DOI：
--
复制
发表时间：
2003
影响因子：
4
通讯作者：
W. Purcell
中科院分区：
文献类型：
--
作者：
Mingwen Ma;Jinsheng Xu;W. Purcell
在分离的单个肝细胞聚集在一起形成三维球体的过程中，细胞经历了巨大的环境变化。肝细胞如何对这些变化作出反应，以前还没有得到很好的研究。这项研究的特点是功能和生化变化过程中肝球体的形成和维持。在6孔板中，通过旋转介导的方法，从雄性Sprague大鼠新鲜分离的肝细胞制备球状体。在培养21天的时间内，对肝球状体的形态形成、功能和生化参数进行了评价。根据其大小和形状以及其功能的变化，将肝球体形成分为两个阶段，未成熟（1-5天）和成熟（>5天）。在整个观察期间，半乳糖和丙酮酸消耗保持在相对稳定的水平。然而，葡萄糖分泌和细胞的GPT和GOT活性较高，在未成熟的球体，下降到第5天，并保持稳定之后。细胞γ-谷氨酰转移酶（γ-GT）和乳酸脱氢酶（LDH）活性最初检测不到或较低，并随着球状体成熟而增加。白蛋白分泌在前2天内迅速下降，并随着球状体成熟而增加。它的结论是，细胞经历功能和生化变化，在球体形成后，从完整的组织中分离肝细胞。功能和生化特性恢复，并保持在成熟的球体。一个相对稳定的时期（6-15天）的功能在成熟的球状体被确定，并建议该模型的应用。© 2003 Wiley利斯公司
In the process of isolated single liver cells coming together to form three‐dimensional spheroids, cells undergo dramatic environmental changes. How liver cells respond to these changes has not been well studied before. This study characterized the functional and biochemical changes during liver spheroid formation and maintenance. Spheroids were prepared in 6‐well plates from freshly isolated liver cells from male Sprague rats by a gyrotatory‐mediated method. Morphological formation, and functional and biochemical parameters of liver spheroids were evaluated over a period of 21 days in culture. Liver spheroid formation was divided into two stages, immature (1–5 days) and mature (>5 days), according to their size and shape, and changes in their functionality. Galactose and pyruvate consumption was maintained at a relatively stable level throughout the period of observation. However, glucose secretion and cellular GPT and GOT activities were higher in immature spheroids, decreased upto day 5 and remained stable thereafter. Cellular γ‐glutamyltransferase (γ‐GT) and lactate dehydrogenase (LDH) activities were initially undetectable or low and increased as spheroids matured. Albumin secretion decreased rapidly within the first 2 days and increased as spheroids matured. It is concluded that cells undergo functional and biochemical changes during spheroid formation following isolation of liver cells from intact tissue. Functionality and biochemical properties recovered and were maintained in mature spheroids. A relatively stable period (6–15 days) of functionality in mature spheroids was identified and is recommended for applications of the model. © 2003 Wiley‐Liss, Inc.
动态肝脏培养系统：研究化学生物转化和毒性的工具。
DOI：
10.1007/978-3-642-72558-6_3
发表时间：
1987
期刊：
Archives of toxicology. Supplement. = Archiv fur Toxikologie. Supplement
影响因子：
--
作者：
Sipes,IG;Fisher,RL;Smith,PF;Stine,ER;Gandolfi,AJ;Brendel,K
通讯作者：
Brendel,K
大鼠肝细胞悬浮液产生的一氧化氮的时间依赖性。
影响因子：
5.8
作者：
Nicholls-Grzemski,FA;Tirmenstein,MA;Fariss,MW
通讯作者：
Fariss,MW