The neuron-astrocyte-microglia triad in normal brain ageing and in a model of neuroinflammation in the rat hippocampus.
The neuron-astrocyte-microglia triad in normal brain ageing and in a model of neuroinflammation in the rat hippocampus.
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DOI：
10.1371/journal.pone.0045250
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Giovannini MG
中科院分区：
文献类型：
--
作者：
Cerbai F;Lana D;Nosi D;Petkova-Kirova P;Zecchi S;Brothers HM;Wenk GL;Giovannini MG
衰老伴随着认知功能的下降；伴随着各种神经生物学变化。炎症和衰老之间的联系是基于复杂的分子和细胞变化，我们才刚刚开始了解。海马是与衰老过程中电生理、结构和形态变化关系更为密切的结构之一。在本研究中，我们研究了正常衰老和lps诱导的炎症对成年、正常衰老和lps治疗的成年大鼠海马星形胶质细胞-神经元相互作用的影响。与成年大鼠和lps处理大鼠相比，老龄大鼠CA1辐射体星形胶质细胞更小，分支更粗，更短，数量更少。星形胶质细胞分支浸润老龄大鼠和lps处理大鼠的凋亡神经元。衰老大鼠和lps处理大鼠的CA1细胞碎片数量更多，可以发现它们与星形胶质细胞过程相对抗，并被反应性小胶质细胞吞噬。反应性小胶质细胞存在于CA1辐射体中，通常与凋亡细胞相关。反应性小胶质细胞的比例在成年大鼠中为40%，老年大鼠为33%，lps处理大鼠为50%，差异有统计学意义。衰老大鼠和lps处理大鼠海马匀浆中Fractalkine （CX3CL1）显著升高。老龄大鼠CA1神经元数量减少。在衰老和lps处理的大鼠海马中，星形胶质细胞和小胶质细胞可能通过CX3CL1信号传导帮助清除凋亡细胞碎片。我们的研究结果表明，老年大鼠和lps注射大鼠海马中的星形胶质细胞和小胶质细胞可能参与了与程序性细胞死亡相关的细胞碎片的清除。星形胶质细胞的作用可能代表了控制炎症过程和进一步细胞损伤向邻近组织扩散的保护机制，或者它们可能在病理条件下促进神经元损伤。
Ageing is accompanied by a decline in cognitive functions; along with a variety of neurobiological changes. The association between inflammation and ageing is based on complex molecular and cellular changes that we are only just beginning to understand. The hippocampus is one of the structures more closely related to electrophysiological, structural and morphological changes during ageing. In the present study we examined the effect of normal ageing and LPS-induced inflammation on astroglia-neuron interaction in the rat hippocampus of adult, normal aged and LPS-treated adult rats. Astrocytes were smaller, with thicker and shorter branches and less numerous in CA1 Str. radiatum of aged rats in comparison to adult and LPS-treated rats. Astrocyte branches infiltrated apoptotic neurons of aged and LPS-treated rats. Cellular debris, which were more numerous in CA1 of aged and LPS-treated rats, could be found apposed to astrocytes processes and were phagocytated by reactive microglia. Reactive microglia were present in the CA1 Str. Radiatum, often in association with apoptotic cells. Significant differences were found in the fraction of reactive microglia which was 40% of total in adult, 33% in aged and 50% in LPS-treated rats. Fractalkine (CX3CL1) increased significantly in hippocampus homogenates of aged and LPS-treated rats. The number of CA1 neurons decreased in aged rats. In the hippocampus of aged and LPS-treated rats astrocytes and microglia may help clearing apoptotic cellular debris possibly through CX3CL1 signalling. Our results indicate that astrocytes and microglia in the hippocampus of aged and LPS-infused rats possibly participate in the clearance of cellular debris associated with programmed cell death. The actions of astrocytes may represent either protective mechanisms to control inflammatory processes and the spread of further cellular damage to neighboring tissue, or they may contribute to neuronal damage in pathological conditions.
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影响因子：
4.2
作者：
Bachstetter, Adam D.;Morganti, Josh M.;Jernberg, Jennifer;Schlunk, Andrea;Mitchell, Staten H.;Brewster, Kaelin W.;Hudson, Charles E.;Cole, Michael J.;Harrison, Jeffrey K.;Bickford, Paula C.;Gemma, Carmelina
通讯作者：
Gemma, Carmelina
影响因子：
16.2
作者：
Bhaskar, Kiran;Konerth, Megan;Kokiko-Cochran, Olga N.;Cardona, Astrid;Ransohoff, Richard M.;Lamb, Bruce T.
通讯作者：
Lamb, Bruce T.
DOI：
10.1073/pnas.95.18.10896
发表时间：
1998-09-01
影响因子：
11.1
作者：
Harrison, JK;Jiang, Y;Feng, LL
通讯作者：
Feng, LL
影响因子：
4.2
作者：
Bhatnagar, M;Cintra, A;Fuxe, K
通讯作者：
Fuxe, K
影响因子：
6.2
作者：
Herber, DL;Maloney, JL;Gordon, MN
通讯作者：
Gordon, MN