Cloning, sequencing and deletion from the chromosome of the gene encoding subunit I of the aa3‐type cytochrome c oxidase of Rhodobacter sphaeroides
Cloning, sequencing and deletion from the chromosome of the gene encoding subunit I of the aa3‐type cytochrome c oxidase of Rhodobacter sphaeroides
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球形红杆菌aa3型细胞色素c氧化酶亚基I编码基因的克隆、测序和染色体缺失
作者：
J. Shapleigh;R. Gennis
The ctaD gene encoding subunit I of the aa3‐type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% identity with that from Paracoccus denitrificans. The ctaD gene was deleted from the chromosome of R. sphaeroides, resulting in a strain that spectroscopically lacks cytochrome a. This strain maintains about 50% of the cytochrome c oxidase activity of the wild‐type strain owing to the presence of an alternate o‐type cytochrome c oxidase. The aa3‐type oxidase was restored by complementing the chromosomal deletion with a plasmid‐borne copy of the CtaD gene. This system is well suited for site‐directed mutagenesis probing of the structure and function of cytochrome c oxidase.
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通过定点诱变将组氨酸轴向配体分配给来自球形红杆菌的 bc1 复合物的细胞色素 bH 和细胞色素 bL 成分。
影响因子：
2.9
作者：
Yun,CH;Crofts,AR;Gennis,RB
通讯作者：
Gennis,RB
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影响因子：
5.6
作者：
Valpuesta,JM;Henderson,R;Frey,TG
通讯作者：
Frey,TG
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DOI：
10.1111/j.1432-1033.1982.tb06667.x
发表时间：
1982
期刊：
European journal of biochemistry
影响因子：
--
作者：
Gennis,RB;Casey,RP;Azzi,A;Ludwig,B
通讯作者：
Ludwig,B
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DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mather,MW;Springer,P;Fee,JA
通讯作者：
Fee,JA
有关原核细胞色素 c 还原酶和细胞色素 c 氧化酶的一些最新进展。
DOI：
10.1016/s0005-2728(05)80260-9
发表时间：
1991
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Gennis,RB
通讯作者：
Gennis,RB