Invasion by WC5 rat cerebellar cells is independent of RSV-induced changes in growth and adhesion.

Invasion by WC5 rat cerebellar cells is independent of RSV-induced changes in growth and adhesion.
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WC5 大鼠小脑细胞的侵袭与 RSV 诱导的生长和粘附变化无关。

DOI:
10.1002/ijc.2910490217
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发表时间:
1991
影响因子:
6.4
通讯作者:
Brackenbury,R
Brackenbury,R
中科院分区:
医学1区
文献类型:
--
作者:
Brady-Kalnay,SM;Boghaert,ER;Zimmer,S;Soll,DR;Brackenbury,R

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The WC5 rat cerebellar cell line, which is infected with a Rous sarcoma virus that is temperature‐sensitive for pp60srctransformation, shows temperature‐dependent expression of the neural‐cell‐adhesion molecule (N‐CAM) and glial fibrillary acidic protein (GFAP). We found that WC5 cells maintained at the non‐permissive temperature in both monolayer cultures and spheroids are subject to density‐dependent inhibition of growth, whereas cells maintained at the permissive temperature continued to grow. The movement of isolated WC5 cells at both temperatures was similar, while the migration of WC5 cells out of 3‐dimensional aggregates was faster at the non‐permissive temperature. We tested whether the RSV‐induced changes affect the invasion of the WC5 cells in 2in vitroassays: the chorio‐allantoic‐membrane assay and the chick‐heart‐fragment assay. In both assays, WC5 cells grown at either temperature were invasive. These results indicate that growth rate is unrelated to invasion and that loss of N‐CAM‐mediated cell‐cell adhesion is not necessary for invasion.
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影响因子: --
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