UV RESISTANCE LOCUS 8 From Chrysanthemum morifolium Ramat (CmUVR8) Plays Important Roles in UV-B Signal Transduction and UV-B-Induced Accumulation of Flavonoids.
UV RESISTANCE LOCUS 8 From Chrysanthemum morifolium Ramat (CmUVR8) Plays Important Roles in UV-B Signal Transduction and UV-B-Induced Accumulation of Flavonoids.
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菊花 Ramat 的抗紫外线位点 8 (CmUVR8) 在 UV-B 信号转导和 UV-B 诱导的黄酮类化合物积累中发挥重要作用
DOI：
10.3389/fpls.2018.00955
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发表时间：
2018
影响因子：
5.6
通讯作者：
Xu M
中科院分区：
文献类型：
--
作者：
Yang Y;Yang X;Jang Z;Chen Z;Ruo X;Jin W;Wu Y;Shi X;Xu M
紫外线抗性基因座8（UV Resistance Locus 8，UVR 8）是一种紫外线-B（UV-B; 280-315 nm）光受体，参与植物生长发育的调控。UV-B辐射是提高药用植物活性成分产量的重要因素。然而，到目前为止，对UV-B光感受器的研究主要集中在拟南芥上，而UVR 8在药用植物中的功能在很大程度上仍然未知。本研究从菊花中分离到一个拟南芥UVR 8的同源物CmUVR 8，并对其结构和功能进行了详细的分析。蛋白质序列分析表明，CmUVR 8与AtUVR 8相似，含有9个染色体浓缩调节子1个重复序列、7个叶片螺旋桨、1个C27区、3个“GWRHT”基序和几个关键氨基酸残基（如14个Trps和2个Arg）。CmUVR 8的三维结构分析表明其结构与AtUVR 8相似。CmUVR 8的异源表达可以挽救拟南芥中UVR 8突变体uvr 8 -6的缺陷表型，表明CmUVR 8在UV-B辐射下下胚轴伸长和HY 5基因表达的调控中起作用。此外，CmUVR 8还调控UV-B诱导的4个类黄酮生物合成相关基因的表达以及UV-B诱导的类黄酮积累。此外，CmUVR 8和CmCOP 1之间的相互作用，证实了使用酵母双杂交试验。这些结果表明CmUVR 8在UV-B信号转导和UV-B诱导的类黄酮积累中起着重要的作用，与AtUVR 8类似。
UV Resistance Locus 8 (UVR8), an ultraviolet-B (UV-B; 280–315 nm) photoreceptor, participates in the regulation of various plant growth and developmental processes. UV-B radiation is an important factor enhancing the production of active components in medicinal plants. To-date, however, studies on UV-B photoreceptors have largely focused on Arabidopsis, and the functions of UVR8 in medicinal plants are still largely unknown. In the present study, a homolog of Arabidopsis UVR8, CmUVR8, was isolated from Chrysanthemum morifolium Ramat, and its structure and function were analyzed in detail. Protein sequence analysis showed that CmUVR8 contained nine conserved regulators of chromosome condensation 1 repeats, seven conserved bladed propellers, one C27 region, three “GWRHT” motifs and several crucial amino acid residues (such as 14 Trps and 2 Args), similar to AtUVR8. 3-D structural analysis of CmUVR8 indicated that its structure was similar to AtUVR8. Heterologous expression of CmUVR8 could rescued the deficient phenotype of uvr8-6, a mutant of UVR8 in Arabidopsis, indicating the role of CmUVR8 in the regulation of hypocotyl elongation and HY5 gene expression under UV-B irradiation. Moreover, CmUVR8 regulates UV-B-induced expression of four flavonoids biosynthesis-related genes and the UV-B-induced accumulation of flavonoids. Furthermore, the interaction between CmUVR8 and CmCOP1 were confirmed using a yeast two-hybrid assay. These results indicated that CmUVR8 plays important roles in UV-B signal transduction and the UV-B-induced accumulation of flavonoids, as a counterpart of AtUVR8.
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影响因子：
5.1
作者：
Boccalandro, HE;Rossi, MC;Casal, JJ
通讯作者：
Casal, JJ
影响因子：
7.2
作者：
Kim, BC;Tennessen, DJ;Last, RL
通讯作者：
Last, RL
影响因子：
4.9
作者：
Correll, MJ;Kiss, JZ
通讯作者：
Kiss, JZ
DOI：
10.1111/tpj.13260
发表时间：
2016-10
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Heilmann M;Velanis CN;Cloix C;Smith BO;Christie JM;Jenkins GI
通讯作者：
Jenkins GI
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF