Immobilization of platinum nanoparticles and glucose oxidase on eggshell membrane for glucose detection
Immobilization of platinum nanoparticles and glucose oxidase on eggshell membrane for glucose detection
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铂纳米颗粒和葡萄糖氧化酶固定在蛋壳膜上用于葡萄糖检测
DOI：
10.1039/c3ay40327k
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发表时间：
2013-09
影响因子：
3.1
通讯作者：
Shuang, Shaomin
中科院分区：
文献类型：
--
作者：
Li, Bo;Dong, Chuan;Choi, Martin M. F.;Shuang, Shaomin
一种新型葡萄糖生物传感器基于铂纳米颗粒（PtNPs）对酶促反应的催化作用，用于葡萄糖测定。 PtNPs 在蛋壳膜 (ESM) 表面原位合成，在蛋壳膜 (ESM) 表面上同时固定有葡萄糖氧化酶 (GOx)，伴随着 PtNPs 的合成，产生 GOx-PtNPs/ESM。通过将 GOx-PtNPs/ESM 放置在溶解氧传感器的表面上来制造葡萄糖生物传感器。通过溶解氧的减少来量化葡萄糖浓度。详细研究了 K2PtCl4 的量 (0.1 M)、GOx 的体积 (1%, w/v)、反应时间、pH、磷酸盐缓冲液浓度和温度对葡萄糖生物传感器响应的影响。该生物传感器表现出小于 30 s 的响应时间、10-225 μM 的宽线性浓度范围和 5 μM 的检测限、良好的重复性（RSD = 1.03%，n = 10）和令人满意的重现性（RSD = 0.54%，n = 5）。在 25 °C 下 8 个月后，生物传感器保留了 90.0% 的初始响应。常见干扰物如D-果糖、柠檬酸、蔗糖、L-抗坏血酸等均未产生明显干扰。所提出的生物传感器成功应用于人体血清中葡萄糖浓度的测定，回收率在93.6%至102.8%之间。
A novel glucose biosensor based on the catalytic effect of platinum nanoparticles (PtNPs) on the enzymatic reaction for the glucose determination has been developed. PtNPs were in-situ synthesized on the surface of the eggshell membrane (ESM) upon which glucose oxidase (GOx) had been simultaneously immobilized accompanied by the synthesis of the PtNPs to produce a GOx-PtNPs/ESM. The glucose biosensor was fabricated by positioning the GOx-PtNPs/ESM on the surface of a dissolved oxygen sensor. The glucose concentration was quantified by the decrease of dissolved oxygen. The effect of the amount of K2PtCl4 (0.1 M), the volume of the GOx (1%, w/v), reaction time, pH, phosphate buffer concentration and temperature on the response of the glucose biosensor were studied in detail. This biosensor exhibited a response time of less than 30 s, a wide linear concentration range of 10–225 μM and a detection limit of 5 μM, good repeatability (RSD = 1.03%, n = 10), and satisfactory reproducibility (RSD = 0.54%, n = 5). The biosensor retained 90.0% of its initial response after 8 months at 25 °C. Common interferents such as D-fructose, citric acid, sucrose, L-ascorbic acid and so on did not any give significant interference. The proposed biosensor was successfully applied for the determination of glucose concentration in human blood serum with recoveries between 93.6% and 102.8%.
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影响因子：
2.9
作者：
Baoli Wu;Guomei Zhang;Yan Zhang;S. Shuang;Martin M. F. Choi
通讯作者：
Baoli Wu;Guomei Zhang;Yan Zhang;S. Shuang;Martin M. F. Choi
影响因子：
1.7
作者：
KUBO, R
通讯作者：
KUBO, R
基于生物相容性羧甲基壳聚糖-金纳米粒子纳米复合物的辣根过氧化物酶的直接电化学
影响因子：
12.6
作者：
Xu, Qin;Mao, Chun;Sheng, Jian
通讯作者：
Sheng, Jian
影响因子：
5.7
作者：
Sheng-Han Wu;Z. Zhong;Dong Wang;Mengxia Li;Y. Qing;N. Dai;Zeng-peng Li
通讯作者：
Sheng-Han Wu;Z. Zhong;Dong Wang;Mengxia Li;Y. Qing;N. Dai;Zeng-peng Li
影响因子：
3
作者：
K. Male;J. Luong
通讯作者：
K. Male;J. Luong