Epidemiological Studies of Pan-Azole Resistant Aspergillus fumigatus Populations Sampled during Tulip Cultivation Show Clonal Expansion with Acquisition of Multi-Fungicide Resistance as Potential Driver.
Epidemiological Studies of Pan-Azole Resistant Aspergillus fumigatus Populations Sampled during Tulip Cultivation Show Clonal Expansion with Acquisition of Multi-Fungicide Resistance as Potential Driver.
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DOI：
10.3390/microorganisms9112379
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发表时间：
2021-11-18
期刊：
影响因子：
4.5
通讯作者：
Lucas JA
中科院分区：
文献类型：
--
作者：
Fraaije BA;Atkins SL;Santos RF;Hanley SJ;West JS;Lucas JA
在临床和环境烟曲霉 (Af) 群体中发现了泛唑抗性分离株。唑类抗药性在这两种情况下都会发生，Af 直接成为患者抗真菌药物的目标，而在环境中，Af 无意中接触到用于材料保存和植物病害控制的杀菌剂。最近报道了对非唑类杀菌剂的耐药性，包括甲基苯并咪唑氨基甲酸酯（MBC）、醌外部抑制剂（QoIs）和琥珀酸脱氢酶抑制剂（SDHI）。这些杀菌剂组不用于医学，但可以在泛唑抗性基因型的进一步传播中发挥重要作用。我们使用杀菌剂敏感性测试和一系列基因分型工具（包括 STRAf 分型和杀菌剂抗性等位基因测序），研究了从郁金香田土壤、郁金香果皮废物和花卉堆肥堆中取样的 Af 种群的多重杀菌剂抗性状况和遗传多样性。郁金香球茎种群中存在两个主要克隆。与临床分离株和文献数据的比较显示，在环境中成功扩增的TR34/L98H和TR46/Y121F/T289A菌株的几个常见克隆谱系也获得了对MBC、QoI和/或SDHI杀菌剂的抗性。携带多种杀菌剂抗性等位基因的菌株在存在属于不同作用模式的多种杀菌剂残留的环境中具有竞争优势。
Pan-azole resistant isolates are found in clinical and environmental Aspergillus fumigatus (Af) populations. Azole resistance can evolve in both settings, with Af directly targeted by antifungals in patients and, in the environment, Af unintendedly exposed to fungicides used for material preservation and plant disease control. Resistance to non-azole fungicides, including methyl benzimidazole carbamates (MBCs), quinone outside inhibitors (QoIs) and succinate dehydrogenase inhibitors (SDHIs), has recently been reported. These fungicide groups are not used in medicine but can play an important role in the further spread of pan-azole resistant genotypes. We investigated the multi-fungicide resistance status and the genetic diversity of Af populations sampled from tulip field soils, tulip peel waste and flower compost heaps using fungicide sensitivity testing and a range of genotyping tools, including STRAf typing and sequencing of fungicide resistant alleles. Two major clones were present in the tulip bulb population. Comparisons with clinical isolates and literature data revealed that several common clonal lineages of TR34/L98H and TR46/Y121F/T289A strains that have expanded successfully in the environment have also acquired resistance to MBC, QoI and/or SDHI fungicides. Strains carrying multiple fungicide resistant alleles have a competitive advantage in environments where residues of multiple fungicides belonging to different modes of action are present.
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影响因子：
11.8
作者：
Hagiwara D;Arai T;Takahashi H;Kusuya Y;Watanabe A;Kamei K
通讯作者：
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影响因子：
4.9
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.9
作者：
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通讯作者：
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