Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides.
Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides.
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通过表达猪圆环病毒 2 衣壳蛋白主要表位的重组腺病毒增强小鼠粘膜免疫，并用胞嘧啶-磷酸-鸟苷寡脱氧核苷酸递送
DOI：
10.4142/jvs.2014.15.3.399
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发表时间：
2014
影响因子：
1.8
通讯作者：
Wang CQ
中科院分区：
文献类型：
--
作者：
Chang HT;He XY;Liu YF;Chen L;Guo QH;Yu QY;Zhao J;Wang XW;Yang X;Wang CQ
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4+ T cells and IFN-γ-producing CD8+ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3+, CD3+CD4+CD8-, and CD3+CD4-CD8+ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.
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影响因子：
6.2
作者：
Bode C;Zhao G;Steinhagen F;Kinjo T;Klinman DM
通讯作者：
Klinman DM
影响因子：
5.5
作者：
Kim, Sol;Jang, Ji-Eun;Chang, Jun
通讯作者：
Chang, Jun
新型疫苗开发策略可诱导粘膜免疫。
影响因子：
6.2
作者：
Fujkuyama Y;Tokuhara D;Kataoka K;Gilbert RS;McGhee JR;Yuki Y;Kiyono H;Fujihashi K
通讯作者：
Fujihashi K
影响因子：
2.4
作者：
Kiupel, M;Stevenson, GW;Mittal, SK
通讯作者：
Mittal, SK
影响因子：
5.4
作者：
Harandi, AM;Eriksson, K;Holmgren, J
通讯作者：
Holmgren, J