CRISPR/Cas9-based discovery of maize transcription factors regulating male sterility and their functional conservation in plants.
CRISPR/Cas9-based discovery of maize transcription factors regulating male sterility and their functional conservation in plants.
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基于CRISPR/Cas9发现调节雄性不育的玉米转录因子及其在植物中的功能保护
DOI：
10.1111/pbi.13590
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发表时间：
2021-09
影响因子：
13.8
通讯作者：
Wan X
中科院分区：
文献类型：
--
作者：
Jiang Y;An X;Li Z;Yan T;Zhu T;Xie K;Liu S;Hou Q;Zhao L;Wu S;Liu X;Zhang S;He W;Li F;Li J;Wan X
鉴定核雄性不育基因并阐明其功能对揭示植物雄性生殖规律和促进其在作物育种中的应用具有重要意义。然而，与拟南芥和水稻相比，玉米GMS基因的发现相对较少，而且对其花药和花粉发育的调控途径知之甚少。通过对玉米自交系B73、郑58和M6007花药11个发育时期的转录组进行测序和分析，鉴定出1100个转录因子（TF）基因在不同发育时期稳定差异表达。其中14个玉米TF基因选择5个TF家族9个类型进行CRISPR/Cas9介导的基因突变，经DNA测序鉴定ZmbHLH 51、ZmbHLH 122、ZmTGA 9 - 1/-2/-3、ZmTGA 10、ZmMYB 84、ZmMYB 33 - 1/-2、ZmPHD 11和ZmLBD 10/27 8个类型的12个基因为玉米新的核不育基因，表型和细胞学分析。值得注意的是，ZmTGA 9 - 1/-2/-3三基因突变体和ZmMYB 33 - 1/-2双基因突变体显示完全雄性不育，但其双基因或单基因突变体显示雄性可育性。ZmLBD 10/27双基因突变体表现部分雄性不育，花粉败育率为32.18%。此外，ZmbHLH 51被ZmbHLH 122转录激活，并且它们的蛋白质存在物理相互作用。开发了与这些GMS突变共分离的分子标记，以促进其在玉米育种中的应用。最后，所有14类型的玉米GMS TF基因在这里和以前报道的玉米，水稻和拟南芥中的功能保守性和多样性进行了比较。这些发现丰富了GMS基因和突变体资源，以深入了解玉米雄性不育的调控网络和创造玉米雄性不育系。
Identifying genic male‐sterility (GMS) genes and elucidating their roles are important to unveil plant male reproduction and promote their application in crop breeding. However, compared with Arabidopsis and rice, relatively fewer maize GMS genes have been discovered and little is known about their regulatory pathways underlying anther and pollen development. Here, by sequencing and analysing anther transcriptomes at 11 developmental stages in maize B73, Zheng58 and M6007 inbred lines, 1100 transcription factor (TF) genes were identified to be stably differentially expressed among different developmental stages. Among them, 14 maize TF genes (9 types belonging to five TF families) were selected and performed CRISPR/Cas9‐mediated gene mutagenesis, and then, 12 genes in eight types, including ZmbHLH51, ZmbHLH122, ZmTGA9‐1/‐2/‐3, ZmTGA10, ZmMYB84, ZmMYB33‐1/‐2, ZmPHD11 and ZmLBD10/27, were identified as maize new GMS genes by using DNA sequencing, phenotypic and cytological analyses. Notably, ZmTGA9‐1/‐2/‐3 triple‐gene mutants and ZmMYB33‐1/‐2 double‐gene mutants displayed complete male sterility, but their double‐ or single‐gene mutants showed male fertility. Similarly, ZmLBD10/27 double‐gene mutant displayed partial male sterility with 32.18% of aborted pollen grains. In addition, ZmbHLH51 was transcriptionally activated by ZmbHLH122 and their proteins were physically interacted. Molecular markers co‐segregating with these GMS mutations were developed to facilitate their application in maize breeding. Finally, all 14‐type maize GMS TF genes identified here and reported previously were compared on functional conservation and diversification among maize, rice and Arabidopsis. These findings enrich GMS gene and mutant resources for deeply understanding the regulatory network underlying male fertility and for creating male‐sterility lines in maize.
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影响因子：
11.6
作者：
Evans, Matthew M. S.
通讯作者：
Evans, Matthew M. S.
影响因子：
12.3
作者：
Čermák T;Baltes NJ;Čegan R;Zhang Y;Voytas DF
通讯作者：
Voytas DF
玉米中高频靶向诱变的农杆菌销售的CRISPR/CAS9系统。
影响因子：
13.8
作者：
Char SN;Neelakandan AK;Nahampun H;Frame B;Main M;Spalding MH;Becraft PW;Meyers BC;Walbot V;Wang K;Yang B
通讯作者：
Yang B
玉米雄性育性和多个物种显性雄性不育系统发育所需的 ZmMs7 分子调控
DOI：
10.1073/pnas.2010255117
发表时间：
2020-09-22
影响因子：
11.1
作者：
An X;Ma B;Duan M;Dong Z;Liu R;Yuan D;Hou Q;Wu S;Zhang D;Liu D;Yu D;Zhang Y;Xie K;Zhu T;Li Z;Zhang S;Tian Y;Liu C;Li J;Yuan L;Wan X
通讯作者：
Wan X
影响因子：
11.4
作者：
Gómez JF;Talle B;Wilson ZA
通讯作者：
Wilson ZA