Molecular regulation of ZmMs7 required for maize male fertility and development of a dominant male-sterility system in multiple species.
Molecular regulation of ZmMs7 required for maize male fertility and development of a dominant male-sterility system in multiple species.
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玉米雄性育性和多个物种显性雄性不育系统发育所需的 ZmMs7 分子调控
DOI：
10.1073/pnas.2010255117
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发表时间：
2020-09-22
影响因子：
11.1
通讯作者：
Wan X
中科院分区：
文献类型：
--
作者：
An X;Ma B;Duan M;Dong Z;Liu R;Yuan D;Hou Q;Wu S;Zhang D;Liu D;Yu D;Zhang Y;Xie K;Zhu T;Li Z;Zhang S;Tian Y;Liu C;Li J;Yuan L;Wan X
建立一个在多物种中有效的雄性不育系统是实现不同植物杂交制种的必要条件，特别是对于没有克隆雄性不育基因的植物。在此，我们确定了玉米雄性不育基因ZmMs7的转录调控机制，从而建立了一个显性的雄性不育系统，该系统已被证明在玉米、水稻和拟南芥中有效。与现有的雄性不育系统相比，该系统具有利用单一转基因盒、不同遗传背景下雄性不育稳定性高、生产荧光转基因和正常色非转基因F1杂交种子等潜在优势，可在不同禁止或允许转基因作物种植的国家灵活使用。因此，它是一种简单、经济、适用于多种作物的生物技术。了解雄性不育的分子基础，开发实用的雄性不育系统，对作物杂种优势利用和商业杂交制种具有重要意义。本文报道了玉米雄性不育基因7 （ZmMs7）的分子调控及其在多种优势雄性不育系统中的应用。ZmMs7在玉米花药中特异表达，编码一种具有转录激活功能的植物同源结构域（PHD）指蛋白，在绒毡层发育和花粉外皮形成中起关键作用。ZmMs7可以与玉米核因子Y （NF-Y）亚基相互作用，形成ZmMs7- nf - ya6 - yb2 - yc9 /12/15蛋白复合物，通过直接结合启动子区域的CCAAT盒子激活靶基因。ZmMs7在玉米中通过花药特异性启动子p5126过早表达，导致雄性不育，但营养生长正常，雌性不育。过早表达ZmMs7诱导的ZmMs7下游基因的早期表达导致p5126-ZmMs7玉米系绒毡层发育和花粉外壁形成异常。p5126-ZmMs7转基因水稻和拟南芥表现出相似的显性雄性不育性。与此同时，mCherry基因与p5126-ZmMs7偶联有助于基于荧光选择的优势不育种子的分选。此外，ms7-6007隐性雄性不育系和p5126-ZmMs7M显性雄性不育系在不同遗传种质条件下均具有较高的稳定性，可用于杂交玉米育种。总之，我们的工作提供了对花药和花粉发育机制的深入了解，并为作物杂交种子生产提供了一种有前途的技术。
Significance Developing a male-sterility system that is effective in multiple species is essential for hybrid seed production in different plants, especially for plants without cloned male-sterility genes. Here, we identified the transcriptional regulation mechanism for maize male-sterility gene ZmMs7 and thereby developed a dominant male-sterility system that was proved to be effective in maize, rice, and Arabidopsis. Compared with current male-sterility systems, this system has potential advantages, e.g., utilization of a single transgene cassette, high stability of male sterility under different genetic backgrounds, and producing fluorescent transgenic and normal color nontransgenic F1 hybrid seeds which can be used flexibly in different countries where transgenic crop cultivation is prohibited or allowed. Therefore, it is a simple, cost-effective, and multiple-crop-applicable biotechnology. Understanding the molecular basis of male sterility and developing practical male-sterility systems are essential for heterosis utilization and commercial hybrid seed production in crops. Here, we report molecular regulation by genic male-sterility gene maize male sterility 7 (ZmMs7) and its application for developing a dominant male-sterility system in multiple species. ZmMs7 is specifically expressed in maize anthers, encodes a plant homeodomain (PHD) finger protein that functions as a transcriptional activator, and plays a key role in tapetal development and pollen exine formation. ZmMs7 can interact with maize nuclear factor Y (NF-Y) subunits to form ZmMs7-NF-YA6-YB2-YC9/12/15 protein complexes that activate target genes by directly binding to CCAAT box in their promoter regions. Premature expression of ZmMs7 in maize by an anther-specific promoter p5126 results in dominant and complete male sterility but normal vegetative growth and female fertility. Early expression of ZmMs7 downstream genes induced by prematurely expressed ZmMs7 leads to abnormal tapetal development and pollen exine formation in p5126-ZmMs7 maize lines. The p5126-ZmMs7 transgenic rice and Arabidopsis plants display similar dominant male sterility. Meanwhile, the mCherry gene coupled with p5126-ZmMs7 facilitates the sorting of dominant sterility seeds based on fluorescent selection. In addition, both the ms7-6007 recessive male-sterility line and p5126-ZmMs7M dominant male-sterility line are highly stable under different genetic germplasms and thus applicable for hybrid maize breeding. Together, our work provides insight into the mechanisms of anther and pollen development and a promising technology for hybrid seed production in crops.
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DOI：
10.1186/s12284-014-0013-6
发表时间：
2014-12
期刊：
Rice (New York, N.Y.)
影响因子：
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影响因子：
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通讯作者：
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影响因子：
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通讯作者：
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