Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process.
Dentinal mineralization is not limited in the mineralization front but occurs along with the entire odontoblast process.
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牙本质矿化并不局限于矿化前沿，而是与整个成牙本质细胞过程一起发生
DOI：
10.7150/ijbs.25712
复制
发表时间：
2018
影响因子：
9.2
通讯作者：
Feng JQ
中科院分区：
文献类型：
--
作者：
Li C;Jing Y;Wang K;Ren Y;Liu X;Wang X;Wang Z;Zhao H;Feng JQ
成矿前沿理论在成矿研究领域有着几十年的历史渊源。这一理论被广泛用于描述骨生成和牙本质生成中的矿化事件。然而，该模型并没有提供足够的证据来解释矿物质如何从髓端牙本质传播到牙本质-牙釉质交界处（DEJ）。为了解决这个问题，我们修改了当前的研究方法：a）将矿物沉积时间窗口从几分钟延长到几小时，而不是仅将矿化测定限制在几天和几周内; B）将常规荧光显微镜切换为更强大的共聚焦显微镜;其中可以记录矿物沉积速率和详细的矿物标记以及牙本质小管;沿着;和c）使用报告小鼠，包括Gli 1-CreERT 2活化的番茄和2.3 Col 1-GFP，以标记成牙本质细胞过程，并结合矿物染料注射。我们的主要发现是：1）成牙本质细胞突起分布均匀，在前牙本质-牙本质交界处突起密度大，主突起直径大; 2）成牙本质细胞突起周围的矿物质存款沿着分布，形成许多独立的矿物质环。因此，这些合并的衣领在牙本质-前牙本质连接处产生单一标记线，其中与其他牙本质区域相比，牙本质小管直径更宽，成牙本质细胞过程更致密。因此，我们认为成牙本质细胞的过程直接参与了矿化过程，而不是简单地局限于牙本质和前牙本质边缘的矿化前沿，而是沿着整个成牙本质细胞的过程。这些新发现将为我们理解牙本质结构和功能以及矿化机制提供新的思路。
The mineralization-front theory is historically rooted in mineralization research fields for many decades. This theory is widely used to describe mineralization events in both osteogenesis and dentinogenesis. However, this model does not provide enough evidence to explain how minerals are propagated from the pulp-end dentin to dentin-enamel junction (DEJ). To address this issue, we modified the current research approaches by a) extending the mineral deposition windows of time from minutes to hours, instead of limiting the mineralization assay on days and weeks only; b) switching a regular fluorescent microscope to a more powerful confocal microscope; in which both mineral deposition rates and detail mineral labeling along with dentin tubules can be documented; and c) using reporter mice, including the Gli1-CreERT2 activated tomato and the 2.3 Col1-GFP to mark odontoblast processes combined with mineral dye injections. Our key findings are: 1) Odontoblast-processes, full of numerous mini-branches, evenly spread to entire dentin matrices with a high density of processes and a large diameter of the main process at the predentin-dentin junction; and 2) The minerals deposit along with entire odontoblast-processes and form many individual mineral collars surrounding odontoblast processes. As a result, these merged collars give rise to a single labeled line at the dentin-predentin junction, in which the dental tubules are wider in diameter and denser in odontoblast processes compared to other dentin areas. We therefore propose that it is the odontoblast-process that directly contributes to mineralization, which is not simply limited in the mineralization front at the edge of dentin and predentin, but occurs along with the entire odontoblast process. These new findings will shed new light on our understanding of dentin structure and function, as well as the mechanisms of mineralization.
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DOI：
10.1083/jcb.56.3.838
发表时间：
1973-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Weinstock M;Leblond CP
通讯作者：
Leblond CP
影响因子：
4.8
作者：
Sreenath, T;Thyagarajan, T;Kulkarni, AB
通讯作者：
Kulkarni, AB
影响因子：
2.7
作者：
Sun, Yao;Chen, Li;Qin, Chunlin
通讯作者：
Qin, Chunlin
影响因子：
2.3
作者：
Yoshiba, K;Yoshiba, N;Ozawa, H
通讯作者：
Ozawa, H
影响因子：
7.6
作者：
MILLER, SC;OMURA, TH;SMITH, LJ
通讯作者：
SMITH, LJ