Regulation of Juvenile Hormone on Summer Diapause of Geleruca daurica and Its Pathway Analysis.
Regulation of Juvenile Hormone on Summer Diapause of Geleruca daurica and Its Pathway Analysis.
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保幼激素对葛花夏季滞育的调控及其通路分析
DOI：
10.3390/insects12030237
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发表时间：
2021-03-11
期刊：
影响因子：
3
通讯作者：
Pang BP
中科院分区：
文献类型：
--
作者：
Ma HY;Li YY;Li L;Tan Y;Pang BP
滞育是昆虫发育过程中的一种休眠状态，在昆虫的生活史中起着重要的作用。据认为，缺乏保幼激素（JH）的结果，生殖滞育发生在成年阶段。然而，我们并不完全了解潜在的分子机制。在本研究中，我们证明了JH类似物甲氧普烯的局部应用引起了大量基因转录水平的变化，抑制了脂质积累，最终延迟了成虫进入滞育。因此，JH信号在调节G.蝙蝠蛾是一种在内蒙古大发生的新害虫。摘要保幼激素（JH）信号在昆虫生殖滞育的调控中起着重要作用。然而，我们很少了解JH在滞育的转录组水平上对基因表达的影响。达乌里韦是内蒙古草原上的一种新害虫，成虫期为夏季滞育。在滞育前期局部施用JH类似物甲氧普烯可延迟成虫进入滞育并抑制脂质积累，而在滞育期间则不然。利用Illumina测序技术和生物信息学工具，在处理后1 d和2 d分别检测到54个和138个差异表达基因（DEG）。KEGG分析表明DEGs主要富集在代谢途径中。qRT-PCR分析表明，烯虫酯促进了卵黄蛋白原、叉头转录因子和Krüppel同源物1基因的表达，而抑制了保幼激素结合蛋白、保幼激素酯酶、保幼激素酸性甲基转移酶、保幼激素环氧化物水解酶和脂肪酸合成酶2基因的表达。这些结果表明，JH信号通路在调控G.达乌里卡。
Simple Summary Diapause is an arrestment state in development, and plays an important role in life history in insects. It has been thought that a lack in juvenile hormone (JH) results in reproductive diapause occurring at the adult stage. However, we do not fully know about the underlying molecular mechanism. In this study, we proved that the topical application of a JH analog methoprene caused the changes at the transcriptional levels of a great number of genes, inhibited lipid accumulation, and finally delayed the adults entering diapause. Therefore, JH signaling plays an important role in regulating reproductive diapause of G. daurica, a new pest with great outbreaks in Inner Mongolia. Abstract Juvenile hormone (JH) signaling plays an important role in regulation of reproductive diapause in insects. However, we have little understanding of the effect of JH on gene expression at the transcriptome level in diapause. Galeruca daurica is a new pest in the Inner Mongolia grasslands with obligatory summer diapause in the adult stage. Topical application of a JH analog methoprene at the pre-diapause stage delayed the adults entering diapause and inhibited lipid accumulation whereas it did not during diapause. Using Illumina sequencing technology and bioinformatics tools, 54 and 138 differentially expressed genes (DEGs) were detected at 1 and 2 d after treatment, respectively. The KEGG analysis showed that the DEGs were mainly enriched in the metabolism pathways. qRT-PCR analysis indicated that methoprene promoted the expression of genes encoding vitellogenin, fork head transcription factor and Krüppel homolog 1, whereas suppressed the expression of genes encoding juvenile hormone-binding protein, juvenile hormone esterase, juvenile hormone acid methyltransferase, juvenile hormone epoxide hydrolase and fatty acid synthase 2. These results indicate that JH signaling plays an important role in regulating reproductive diapause of G. daurica.
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影响因子：
4
作者：
Kikuta S;Hagiwara-Komoda Y;Noda H;Kikawada T
通讯作者：
Kikawada T
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
4
作者：
Cheng, Wei-Ning;Li, Xue-Jiao;Zhu-Salzman, Keyan
通讯作者：
Zhu-Salzman, Keyan
影响因子：
4.8
作者：
Kim, Il Hwan;Van Pham;Andersen, John F.
通讯作者：
Andersen, John F.
Krüppel-同源物 1 的分子特征及其在白背飞虱（半翅目：飞虱科）卵巢发育中的作用
影响因子：
2.8
作者：
Hu, Kui;Tian, Ping;Li, Youzhi
通讯作者：
Li, Youzhi